Folate and vitamin B12 improved alcohol-induced hyperhomocysteinemia in rats

Ya Ling Chen, Sien Sing Yang, Hsiang Chi Peng, Yi Ching Hsieh, Jiun-Rong Chen, Suh-Ching Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: The purpose of this study was to investigate the protective effects of combined treatment of folate and vitamin B12 against alcoholic liver disease. Methods: Male Wistar rats weighing about 160 g were divided into four groups: an ethanol group fed an ethanol liquid diet; a control group pair-fed an isoenergetic diet without ethanol; an ethanol and vitamin group fed an ethanol-containing diet that was supplemented with folate (10 mg/kg of body weight per day) and vitamin B12 (0.5 mg/kg of body weight per day); and a control and vitamin group fed an isoenergetic diet without ethanol, which was supplemented with folate (10 mg/kg of body weight per day) and vitamin B12 (0.5 mg/kg of body weight per day). Results: After 16 wk, the plasma folate concentration in the ethanol group was significantly lower than in the other three groups. The plasma homocysteine concentration in the ethanol group was significantly higher than in the other three groups. The hepatic matrix metalloproteinase-2 concentration in the ethanol group was significantly higher than in the control and ethanol/vitamin groups. Furthermore, the plasma homocysteine concentration at the 16th week and the hepatic matrix metalloproteinase-2 concentration showed a significant positive correlation in rats of each group. In addition, pathologic evidence of liver fibrosis was observed only in the ethanol group. Furthermore, hepatic cytochrome 2E1 protein expression in group E increased significantly. Conclusion: These results suggest that combined treatment of folate and vitamin B12 can alleviate alcoholic liver injury that may be related to normalization of plasma homocysteine levels.

Original languageEnglish
Pages (from-to)1034-1039
Number of pages6
JournalNutrition
Volume27
Issue number10
DOIs
Publication statusPublished - Oct 2011

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Keywords

  • Alcoholic liver disease
  • Fibrosis
  • Folate
  • Homocysteine
  • Vitamin B12

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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