Fluoxetine inhibits DNA repair and NF-ĸB-modulated metastatic potential in non-small cell lung cancer

Jeng Yuan Wu, Song Shei Lin, Fei Ting Hsu, Jing Gung Chung

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background/Aim: The aim of present study was to verify the effect of fluoxetine on DNA repair and metastatic potential in non-small cell lung cancer (NSCLC) in vitro. Materials and Methods: Highly metastatic NSCLC CL1-5-F4 cells were used in this study. Cells were treated with different concentrations of fluoxetine or QNZ (NF-ĸB inhibitor) for 48 h. After treatment, cell viability, apoptotic signaling, NF-ĸB activation, expression of DNA repair and metastasis-associated proteins, and cell migration/invasion were evaluated by (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay, flow cytometry, NF-ĸB reporter gene, western blotting, and cell migration/invasion assay, respectively. Results: Fluoxetine induced apoptosis and reduced cell viability, NF-ĸB activation, expression of DNA repair and metastasis-associated proteins, and cell migration/invasion in CL1-5-F4 cells. Also, NF-ĸB activation was the critical factor in fluoxetine-inhibited metastatic potential. Conclusion: Fluoxetine induced apoptosis and inhibited DNA repair and metastatic potential in NSCLC CL1-5-F4 cells.

Original languageEnglish
Pages (from-to)5201-5210
Number of pages10
JournalAnticancer Research
Volume38
Issue number9
DOIs
Publication statusPublished - Sep 1 2018

Keywords

  • DNA repair
  • Fluoxetine
  • Metastatic potential
  • NF-ĸB
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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