Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice

Chien Ming Chen, Yih Huei Uen, Chen Yi Kuo, Tzu Chuan Huang, Jen Ai Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We determined the concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of normal and aristolochic acid (AA)-treated mice. The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically by column-switching high-performance liquid chromatography (HPLC). Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for enantiomeric separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

Original languageEnglish
Title of host publicationCommunications in Computer and Information Science
Pages63-68
Number of pages6
Volume57 CCIS
DOIs
Publication statusPublished - 2009
EventInternational Conference on Bio-Science and Bio-Technology, BSBT 2009. Held as Part of the Future Generation Information Technology Conference, FGIT 2009 - Jeju Island, Korea, Republic of
Duration: Dec 10 2009Dec 12 2009

Publication series

NameCommunications in Computer and Information Science
Volume57 CCIS
ISSN (Print)18650929

Other

OtherInternational Conference on Bio-Science and Bio-Technology, BSBT 2009. Held as Part of the Future Generation Information Technology Conference, FGIT 2009
CountryKorea, Republic of
CityJeju Island
Period12/10/0912/12/09

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Keywords

  • column-switching HPLC
  • fluorimetric determination
  • L-3-hydroxybutyrate
  • renal damage

ASJC Scopus subject areas

  • Computer Science(all)

Cite this

Chen, C. M., Uen, Y. H., Kuo, C. Y., Huang, T. C., & Lee, J. A. (2009). Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. In Communications in Computer and Information Science (Vol. 57 CCIS, pp. 63-68). (Communications in Computer and Information Science; Vol. 57 CCIS). https://doi.org/10.1007/978-3-642-10616-3_9