Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs

Long Jyun Su, Meng Shiue Wu, Yuen Yung Hui, Be Ming Chang, Lei Pan, Pei Chen Hsu, Yit Tsong Chen, Hong Nerng Ho, Yen Hua Huang, Thai Yen Ling, Hsao Hsun Hsu, Huan Cheng Chang

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26 Citations (Scopus)

Abstract

Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.

Original languageEnglish
Article number45607
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Mar 30 2017

ASJC Scopus subject areas

  • General

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    Su, L. J., Wu, M. S., Hui, Y. Y., Chang, B. M., Pan, L., Hsu, P. C., Chen, Y. T., Ho, H. N., Huang, Y. H., Ling, T. Y., Hsu, H. H., & Chang, H. C. (2017). Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs. Scientific Reports, 7, [45607]. https://doi.org/10.1038/srep45607