Fluorescence-guided probes of aptamer-targeted gold nanoparticles with computed tomography imaging accesses for in vivo tumor resection

Cheng Hung Li, Tsung Rong Kuo, Hsin Jan Su, Wei Yun Lai, Pan Chyr Yang, Jinn Shiun Chen, Di Yan Wang, Yi Chun Wu, Chia Chun Chen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Recent development of molecular imaging probes for fluorescence-guided surgery has shown great progresses for determining tumor margin to execute the tissue resection. Here we synthesize the fluorescent gold nanoparticles conjugated with diatrizoic acid and nucleolin-targeted AS1411 aptamer. The nanoparticle conjugates exhibit high water-solubility, good biocompatibility, visible fluorescence and strong X-ray attenuation for computed tomography (CT) contrast enhancement. The fluorescent nanoparticle conjugates are applied as a molecular contrast agent to reveal the tumor location in CL1-5 tumor-bearing mice by CT imaging. Furthermore, the orange-red fluorescence emitting from the conjugates in the CL1-5 tumor can be easily visualized by the naked eyes. After the resection, the IVIS measurements show that the fluorescence signal of the nanoparticle conjugates in the tumor is greatly enhanced in comparison to that in the controlled experiment. Our work has shown potential application of functionalized nanoparticles as a dual-function imaging agent in clinical fluorescence-guided surgery.

Original languageEnglish
Article number15675
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Oct 28 2015

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Gold
Nanoparticles
Fluorescence
Tomography
Neoplasms
Molecular Probes
Molecular Imaging
X Ray Computed Tomography
Solubility
Contrast Media
Water

ASJC Scopus subject areas

  • General

Cite this

Fluorescence-guided probes of aptamer-targeted gold nanoparticles with computed tomography imaging accesses for in vivo tumor resection. / Li, Cheng Hung; Kuo, Tsung Rong; Su, Hsin Jan; Lai, Wei Yun; Yang, Pan Chyr; Chen, Jinn Shiun; Wang, Di Yan; Wu, Yi Chun; Chen, Chia Chun.

In: Scientific Reports, Vol. 5, 15675, 28.10.2015.

Research output: Contribution to journalArticle

Li, Cheng Hung ; Kuo, Tsung Rong ; Su, Hsin Jan ; Lai, Wei Yun ; Yang, Pan Chyr ; Chen, Jinn Shiun ; Wang, Di Yan ; Wu, Yi Chun ; Chen, Chia Chun. / Fluorescence-guided probes of aptamer-targeted gold nanoparticles with computed tomography imaging accesses for in vivo tumor resection. In: Scientific Reports. 2015 ; Vol. 5.
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