FLT3-TKD mutation in childhood acute myeloid leukemia

D. C. Liang, L. Y. Shih, I. J. Hung, C. P. Yang, S. H. Chen, T. H. Jaing, H. C. Liu, L. Y. Wang, W. H. Chang

Research output: Contribution to journalArticle

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Abstract

Mutations of receptor tyrosine kinases are implicated in the constitutive activation and development of human hematologic malignancies. An internal tandem duplication (ITD) of the juxtamembrane domain-coding sequence of the FLT3 gene (FLT3-ITD) is found in 20-25% of adult acute myeloid leukemia (AML) and at a lower frequency in childhood AML. FLT3-ITD is associated with leukocytosis and a poor prognosis, especially in patients with normal karyotype. Recently, there have been three reports on point mutations at codon 835 of the FLT3 gene (D835 mutations) in adult AML. These mutations are located in the activation loop of the second tyrosine kinase domain (TKD) of FLT3 (FLT3-TKD). The clinical and prognostic relevance of the TKD mutations is less clear. To the best of our knowledge, there has been no report to describe FLT3-TKD mutations in childhood AML. In this pediatric series, FLT3-TKD mutations occurred in three of 91 patients (3.3%), an incidence significantly lower than that of FLT3-ITD (14 of 91 patients, 15.4%) in the same cohort of patients. None of them had both FLT3-TKD and FLT3-ITD mutations. Sequence analysis showed one each of D835 Y, D835 V, and D835 H. Of the three patients carrying FLT3-TKD, two had AML-M3 with one each of L- and V-type PML-RARα, and another one had AML-M2 with AML1-ETO. None of our patients with FLT3-TKD had leukocytosis at diagnosis. At bone marrow relapse, one of the four patients examined acquired FLT3-ITD mutation and none gained FLT3-TKD mutation.

Original languageEnglish
Pages (from-to)883-886
Number of pages4
JournalLeukemia
Volume17
Issue number5
DOIs
Publication statusPublished - May 1 2003
Externally publishedYes

Fingerprint

Acute Myeloid Leukemia
Protein-Tyrosine Kinases
Mutation
Leukocytosis
Acute Promyelocytic Leukemia
Receptor Protein-Tyrosine Kinases
Human Development
Hematologic Neoplasms
Karyotype
Point Mutation
Codon
Genes
Sequence Analysis
Bone Marrow
Pediatrics
Recurrence
Incidence

Keywords

  • Childhood acute myeloid leukemia
  • D835 mutation
  • FLT3-ITD
  • FLT3-TKD

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Liang, D. C., Shih, L. Y., Hung, I. J., Yang, C. P., Chen, S. H., Jaing, T. H., ... Chang, W. H. (2003). FLT3-TKD mutation in childhood acute myeloid leukemia. Leukemia, 17(5), 883-886. https://doi.org/10.1038/sj.leu.2402928

FLT3-TKD mutation in childhood acute myeloid leukemia. / Liang, D. C.; Shih, L. Y.; Hung, I. J.; Yang, C. P.; Chen, S. H.; Jaing, T. H.; Liu, H. C.; Wang, L. Y.; Chang, W. H.

In: Leukemia, Vol. 17, No. 5, 01.05.2003, p. 883-886.

Research output: Contribution to journalArticle

Liang, DC, Shih, LY, Hung, IJ, Yang, CP, Chen, SH, Jaing, TH, Liu, HC, Wang, LY & Chang, WH 2003, 'FLT3-TKD mutation in childhood acute myeloid leukemia', Leukemia, vol. 17, no. 5, pp. 883-886. https://doi.org/10.1038/sj.leu.2402928
Liang DC, Shih LY, Hung IJ, Yang CP, Chen SH, Jaing TH et al. FLT3-TKD mutation in childhood acute myeloid leukemia. Leukemia. 2003 May 1;17(5):883-886. https://doi.org/10.1038/sj.leu.2402928
Liang, D. C. ; Shih, L. Y. ; Hung, I. J. ; Yang, C. P. ; Chen, S. H. ; Jaing, T. H. ; Liu, H. C. ; Wang, L. Y. ; Chang, W. H. / FLT3-TKD mutation in childhood acute myeloid leukemia. In: Leukemia. 2003 ; Vol. 17, No. 5. pp. 883-886.
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abstract = "Mutations of receptor tyrosine kinases are implicated in the constitutive activation and development of human hematologic malignancies. An internal tandem duplication (ITD) of the juxtamembrane domain-coding sequence of the FLT3 gene (FLT3-ITD) is found in 20-25{\%} of adult acute myeloid leukemia (AML) and at a lower frequency in childhood AML. FLT3-ITD is associated with leukocytosis and a poor prognosis, especially in patients with normal karyotype. Recently, there have been three reports on point mutations at codon 835 of the FLT3 gene (D835 mutations) in adult AML. These mutations are located in the activation loop of the second tyrosine kinase domain (TKD) of FLT3 (FLT3-TKD). The clinical and prognostic relevance of the TKD mutations is less clear. To the best of our knowledge, there has been no report to describe FLT3-TKD mutations in childhood AML. In this pediatric series, FLT3-TKD mutations occurred in three of 91 patients (3.3{\%}), an incidence significantly lower than that of FLT3-ITD (14 of 91 patients, 15.4{\%}) in the same cohort of patients. None of them had both FLT3-TKD and FLT3-ITD mutations. Sequence analysis showed one each of D835 Y, D835 V, and D835 H. Of the three patients carrying FLT3-TKD, two had AML-M3 with one each of L- and V-type PML-RARα, and another one had AML-M2 with AML1-ETO. None of our patients with FLT3-TKD had leukocytosis at diagnosis. At bone marrow relapse, one of the four patients examined acquired FLT3-ITD mutation and none gained FLT3-TKD mutation.",
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