Flow-cytometric DNA content analysis of oesophageal carcinoma. Comparison between tumour and sequential non-tumour mucosae

Liang Shun Wang, Li Hwa Wu, Chun Ju Chang, Wing Yin Li, Huei Jyh Fahn, Min Hsiung Huang, Jen Hwey Chiu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The DNA content in oesophageal carcinoma and in sequential non-tumour mucosa was evaluated in 35 patients with oesophageal carcinoma, to explore the hypotheses that DNA distribution pattern and S-phase fraction can reflect malignant potential and that DNA aneuploidy can provide an early-warning signal of developing cancer. DNA flow cytometry was performed on 129 specimens from the tumours and on 119 specimens from non-tumour mucosa. Control specimens from gastric fundus had normal diploid DNA content and low S-phase fraction. Aneuploidy was found in 94.3% of the carcinoma specimens and intratumoral heterogeneity in 54.3%. Of the non-tumour specimens, 43.7% showed aneuploidy and none multiple aneuploidy. Pattern III distribution was present in 8.6% of the tumour specimens but not in non-tumour mucosa, where the incidence of aneuploidy rose with closeness to the tumour (p <0.001). S-phase fraction was smaller in non-tumour than in tumour specimens (p <0.0001). The study indicated that histologically tumour-free oesophageal mucosa may have a high malignant potential in patients with oesophageal carcinoma. The relative instability of such mucosa, with aneuploid cells and low S-phase fraction, may facilitate transition to abnormally proliferating cells in response to environmental signals. Cigarette smoking and alcohol may increase the risk of multicentric cancer development.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalScandinavian Cardiovascular Journal
Volume32
Issue number4
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Aneuploidy
Mucous Membrane
Carcinoma
S Phase
DNA
Neoplasms
Gastric Fundus
Diploidy
Flow Cytometry
Smoking
Alcohols
Incidence

Keywords

  • DNA content analysis
  • Flow cytometry
  • Oesophageal cancer

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Flow-cytometric DNA content analysis of oesophageal carcinoma. Comparison between tumour and sequential non-tumour mucosae. / Wang, Liang Shun; Wu, Li Hwa; Chang, Chun Ju; Li, Wing Yin; Fahn, Huei Jyh; Huang, Min Hsiung; Chiu, Jen Hwey.

In: Scandinavian Cardiovascular Journal, Vol. 32, No. 4, 1998, p. 205-212.

Research output: Contribution to journalArticle

Wang, Liang Shun ; Wu, Li Hwa ; Chang, Chun Ju ; Li, Wing Yin ; Fahn, Huei Jyh ; Huang, Min Hsiung ; Chiu, Jen Hwey. / Flow-cytometric DNA content analysis of oesophageal carcinoma. Comparison between tumour and sequential non-tumour mucosae. In: Scandinavian Cardiovascular Journal. 1998 ; Vol. 32, No. 4. pp. 205-212.
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