Flow Cytometric Detection of Mitochondrial Membrane Potential

Hsin-Yi Chang, Hsuan-Cheng Huang, Tsui-Chin Huang, Pan-Chyr Yang, Yi-Ching Wang, Hsueh-Fen Juan

Research output: Book/ReportBook

Abstract

Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. Recently, lipophilic cationic fluorescent dyes have been developed to detect Δψm by accumulating in the mitochondrial matrix until the Nernstian equilibrium distribution of lipophilic cations is reached. In this protocol, we applied a cell-permeant, green-fluorescent, lipophilic dye 3,3'-dihexyloxacarbocyanine Iodide (DiOC6(3)) which accumulates in mitochondria due to their large negative membrane potential, it can be applied to monitor the mitochondrial membrane potential using flow cytometric detection.
Original languageTraditional Chinese
Volume3
DOIs
Publication statusPublished - Jan 1 2013

Cite this

Flow Cytometric Detection of Mitochondrial Membrane Potential. / Chang, Hsin-Yi; Huang, Hsuan-Cheng; Huang, Tsui-Chin; Yang, Pan-Chyr; Wang, Yi-Ching; Juan, Hsueh-Fen.

2013.

Research output: Book/ReportBook

Chang, Hsin-Yi ; Huang, Hsuan-Cheng ; Huang, Tsui-Chin ; Yang, Pan-Chyr ; Wang, Yi-Ching ; Juan, Hsueh-Fen. / Flow Cytometric Detection of Mitochondrial Membrane Potential. 2013.
@book{06f68110b8ca406eb09786a1fedc2c1e,
title = "Flow Cytometric Detection of Mitochondrial Membrane Potential",
abstract = "Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. Recently, lipophilic cationic fluorescent dyes have been developed to detect Δψm by accumulating in the mitochondrial matrix until the Nernstian equilibrium distribution of lipophilic cations is reached. In this protocol, we applied a cell-permeant, green-fluorescent, lipophilic dye 3,3'-dihexyloxacarbocyanine Iodide (DiOC6(3)) which accumulates in mitochondria due to their large negative membrane potential, it can be applied to monitor the mitochondrial membrane potential using flow cytometric detection.",
author = "Hsin-Yi Chang and Hsuan-Cheng Huang and Tsui-Chin Huang and Pan-Chyr Yang and Yi-Ching Wang and Hsueh-Fen Juan",
year = "2013",
month = "1",
day = "1",
doi = "10.21769/BioProtoc.430",
language = "繁體中文",
volume = "3",

}

TY - BOOK

T1 - Flow Cytometric Detection of Mitochondrial Membrane Potential

AU - Chang, Hsin-Yi

AU - Huang, Hsuan-Cheng

AU - Huang, Tsui-Chin

AU - Yang, Pan-Chyr

AU - Wang, Yi-Ching

AU - Juan, Hsueh-Fen

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. Recently, lipophilic cationic fluorescent dyes have been developed to detect Δψm by accumulating in the mitochondrial matrix until the Nernstian equilibrium distribution of lipophilic cations is reached. In this protocol, we applied a cell-permeant, green-fluorescent, lipophilic dye 3,3'-dihexyloxacarbocyanine Iodide (DiOC6(3)) which accumulates in mitochondria due to their large negative membrane potential, it can be applied to monitor the mitochondrial membrane potential using flow cytometric detection.

AB - Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. Recently, lipophilic cationic fluorescent dyes have been developed to detect Δψm by accumulating in the mitochondrial matrix until the Nernstian equilibrium distribution of lipophilic cations is reached. In this protocol, we applied a cell-permeant, green-fluorescent, lipophilic dye 3,3'-dihexyloxacarbocyanine Iodide (DiOC6(3)) which accumulates in mitochondria due to their large negative membrane potential, it can be applied to monitor the mitochondrial membrane potential using flow cytometric detection.

U2 - 10.21769/BioProtoc.430

DO - 10.21769/BioProtoc.430

M3 - 書籍

VL - 3

BT - Flow Cytometric Detection of Mitochondrial Membrane Potential

ER -