Abstract

A novel approach was undertaken to create a potential skin wound dressing. L929 fibroblast cells and alginate solution were simultaneously dispensed into a calcium chloride solution using a three-dimensional plotting system to manufacture a fibrous alginate scaffold with interconnected pores. These cells were then embedded in the alginate hydrogel fibers of the scaffold. A conventional scaffold with cells directly seeded on the fiber surface was used as a control. The encapsulated fibroblasts made using the co-dispensing method distributed homogeneously within the scaffold and showed the delayed formation of large cell aggregates compared to the control. The cells embedded in the hydrogel fibers also deposited more type I collagen in the extracellular matrix and expressed higher levels of fgf11 and fn1 than the control, indicating increased cellular proliferation and attachment. The results indicate that the novel co-dispensing alginate scaffold may promote skin regeneration better than the conventional directly-seeded scaffold.

Original languageEnglish
Pages (from-to)259-269
Number of pages11
JournalJournal of materials science. Materials in medicine
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Tissue Scaffolds
Hydrogel
Alginate
Hydrogels
Scaffolds
Skin
Repair
Fibers
Fibroblasts
Scaffolds (biology)
Cells
Calcium Chloride
Calcium chloride
Collagen Type I
Collagen
Bandages
Extracellular Matrix
Regeneration
Cell Proliferation
alginic acid

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Fibrous hydrogel scaffolds with cells embedded in the fibers as a potential tissue scaffold for skin repair. / Lin, Hsin Yi; Peng, Chih Wei; Wu, Wei Wen.

In: Journal of materials science. Materials in medicine, Vol. 25, No. 1, 01.2014, p. 259-269.

Research output: Contribution to journalArticle

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