Fetal striatal transplants restore electrophysiological sensitivity to dopamine in the lesioned striatum of rats with experimental Huntington's disease

Guann Juh Chen, Churn Hueih Jeng, S. Z. Lin, Shin-Han Tsai, Yun Wang, Yung-Hsiao Chiang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Dopamine (DA), a major neurotransmitter used in the striatum, is involved in movement disorders such as Parkinson's disease and Huntington's chorea. With the loss of neurons in the striatum of patients with Huntington's disease (HD), there is an associated downregulation of DA receptors, which may alter DA-mediated responses. In the present study, DA-mediated electrophysiological depression was studied in animals with quinolinic acid (QA)-induced experimental HD. QA was directly applied to the right striatum of adult female Sprague-Dawley rats. Animals receiving QA developed ipsilateral rotation after the application of apomorphine. Fetal striatal tissue transplants grafted 1 month after lesioning attenuated apomorphine-induced rotation. Six months after lesioning, the animals were anesthetized with urethane for electrophysiological study. DA, applied directly to neurons by pressure microejection, inhibited spontaneous single-unit activity in the striatal neurons of nonlesioned, lesioned and lesioned/grafted rats. QA lesioning reduced responses to DA in the striatal neurons. The dose of DA required to inhibit striatal neuron activity in the lesioned rats was significantly increased compared to that in the nonlesioned rats. Transplantation of fetal striatal tissue restored the electrophysiological sensitivity to DA in the lesioned striatum. The dose of DA used to suppress striatal neuron activity was reduced after grafting. Immunohistostaining showed survival of γ-aminobutyric acid neurons at the graft site. Tyrosine hydroxylase-positive terminals were found innervating the striatal grafts. In conclusion, our data demonstrate that fetal striatal transplants restore electrophysiological sensitivity to DA in the lesioned striatum of animals with experimental HD.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalJournal of Biomedical Science
Volume9
Issue number4
DOIs
Publication statusPublished - Jul 2002

Fingerprint

Corpus Striatum
Transplants
Huntington Disease
Rats
Dopamine
Neurons
Quinolinic Acid
Animals
Apomorphine
Grafts
Fetal Tissue Transplantation
Tissue
Aminobutyrates
Urethane
Dopamine Receptors
Movement Disorders
Tyrosine 3-Monooxygenase
Neurotransmitter Agents
Sprague Dawley Rats
Parkinson Disease

Keywords

  • Dopamine
  • Electrophysiology
  • Huntington's disease
  • Neural transplantation
  • Regeneration
  • Striatum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fetal striatal transplants restore electrophysiological sensitivity to dopamine in the lesioned striatum of rats with experimental Huntington's disease. / Chen, Guann Juh; Jeng, Churn Hueih; Lin, S. Z.; Tsai, Shin-Han; Wang, Yun; Chiang, Yung-Hsiao.

In: Journal of Biomedical Science, Vol. 9, No. 4, 07.2002, p. 303-310.

Research output: Contribution to journalArticle

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