Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori

A kinetic analysis

Hui Er Wang, Chi Huang Chang, Yaw Bee Ker, Chiung Chi Peng, Kuan Chou Chen, Robert Y. Peng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.

Original languageEnglish
Pages (from-to)6730-6739
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number12
DOIs
Publication statusPublished - Jun 22 2011

Fingerprint

peptic ulcers
Proton Pumps
proton pump
Urease
Helicobacter pylori
urease
Peptic Ulcer
Soybeans
soybeans
kinetics
Kinetics
liquids
Liquids
Ulcer
Stomach
stomach
Essential Amino Acids
Fluids
Thiobarbituric Acid Reactive Substances
Testing

Keywords

  • fermented soybean liquid
  • H. pylori urease
  • isoflavonoids
  • peptic ulcer
  • proton pump

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

@article{52257244d4cb434ca06254061a4a9d20,
title = "Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis",
abstract = "Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82{\%} in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6{\%}, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6{\%}, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.",
keywords = "fermented soybean liquid, H. pylori urease, isoflavonoids, peptic ulcer, proton pump",
author = "Wang, {Hui Er} and Chang, {Chi Huang} and Ker, {Yaw Bee} and Peng, {Chiung Chi} and Chen, {Kuan Chou} and Peng, {Robert Y.}",
year = "2011",
month = "6",
day = "22",
doi = "10.1021/jf200770n",
language = "English",
volume = "59",
pages = "6730--6739",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
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TY - JOUR

T1 - Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori

T2 - A kinetic analysis

AU - Wang, Hui Er

AU - Chang, Chi Huang

AU - Ker, Yaw Bee

AU - Peng, Chiung Chi

AU - Chen, Kuan Chou

AU - Peng, Robert Y.

PY - 2011/6/22

Y1 - 2011/6/22

N2 - Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.

AB - Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.

KW - fermented soybean liquid

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KW - proton pump

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