Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture

Guo Shiang Huang, Ting Chen Tseng, Niann Tzyy Dai, Keng Yen Fu, Lien Guo Dai, Shan Hui Hsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10mL to 5mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1×107cells takes about seven days from 5mL of adipose tissue, compared to 14 days using the conventional PA method from 10mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture.

Original languageEnglish
Pages (from-to)154-162
Number of pages9
JournalBiomaterials
Volume65
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Adult Stem Cells
Chitosan
Stem cells
Cell culture
Adipose Tissue
Tissue
Plastics
Cell Aging
Cell Communication

Keywords

  • Adipose-derived adult stem cells (ASCs)
  • CD271
  • Chitosan
  • Plastic adherence (PA)

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture. / Huang, Guo Shiang; Tseng, Ting Chen; Dai, Niann Tzyy; Fu, Keng Yen; Dai, Lien Guo; Hsu, Shan Hui.

In: Biomaterials, Vol. 65, 01.01.2015, p. 154-162.

Research output: Contribution to journalArticle

Huang, Guo Shiang ; Tseng, Ting Chen ; Dai, Niann Tzyy ; Fu, Keng Yen ; Dai, Lien Guo ; Hsu, Shan Hui. / Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture. In: Biomaterials. 2015 ; Vol. 65. pp. 154-162.
@article{1de88a7d4623496eb04fab724da683e7,
title = "Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture",
abstract = "Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10mL to 5mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1×107cells takes about seven days from 5mL of adipose tissue, compared to 14 days using the conventional PA method from 10mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture.",
keywords = "Adipose-derived adult stem cells (ASCs), CD271, Chitosan, Plastic adherence (PA)",
author = "Huang, {Guo Shiang} and Tseng, {Ting Chen} and Dai, {Niann Tzyy} and Fu, {Keng Yen} and Dai, {Lien Guo} and Hsu, {Shan Hui}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2015.07.003",
language = "English",
volume = "65",
pages = "154--162",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Science Ltd",

}

TY - JOUR

T1 - Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture

AU - Huang, Guo Shiang

AU - Tseng, Ting Chen

AU - Dai, Niann Tzyy

AU - Fu, Keng Yen

AU - Dai, Lien Guo

AU - Hsu, Shan Hui

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10mL to 5mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1×107cells takes about seven days from 5mL of adipose tissue, compared to 14 days using the conventional PA method from 10mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture.

AB - Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10mL to 5mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1×107cells takes about seven days from 5mL of adipose tissue, compared to 14 days using the conventional PA method from 10mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture.

KW - Adipose-derived adult stem cells (ASCs)

KW - CD271

KW - Chitosan

KW - Plastic adherence (PA)

UR - http://www.scopus.com/inward/record.url?scp=84937400410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937400410&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2015.07.003

DO - 10.1016/j.biomaterials.2015.07.003

M3 - Article

C2 - 26162932

AN - SCOPUS:84937400410

VL - 65

SP - 154

EP - 162

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -