Fascin is a circulating tumor marker for head and neck cancer as determined by a proteomic analysis of interstitial fluid from the tumor microenvironment

Joseph Tung Chieh Chang, Li Yu Lee, Yin Ju Chen, Ya Ching Lu, Chun Ta Liao, I. How Chen, Yu Chen Huang, Wen Ho Chen, Chi Che Huang, Chi Ying Tsai, Ann Joy Cheng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Head and neck cancer (HNC) is a prevalent cancer worldwide; however, clinically useful tumor markers for HNC have not been identified. Here, we aimed to identify secretory proteins from the tumor microenvironment as candidate circulating tumor markers. Methods: Samples derived from seven pairs of tumor interstitial fluid (TIF) and normal interstitial fluid (NIF) samples from patients with HNC were analyzed. The proteomes were determined by gel-based-mass-spectrometry proteomic methods. The most up-regulated protein, fascin was confirmed in the cancer tissues and cell culture supernatant by immunoblotting and immunohistochemistry assays. Serum fascin was determined in 40 HNC and 40 normal individuals by ELISA. Results: After proteomics analysis, 189 peptides were identified, corresponding to 75 proteins. Of the 21 proteins which were identified more than twice, five up-regulated proteins identified most frequently including fascin. The most elevated fascin was over-expressed in cancer tissues and cell culture supernatant. Serum fascin was significantly up-regulated in the cancer patients (p

Original languageEnglish
Pages (from-to)1631-1641
Number of pages11
JournalClinical Chemistry and Laboratory Medicine
Volume53
Issue number10
DOIs
Publication statusPublished - Sep 1 2015
Externally publishedYes

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Tumor Microenvironment
Extracellular Fluid
Tumor Biomarkers
Head and Neck Neoplasms
Proteomics
Tumors
Fluids
Tissue culture
Neoplasms
Proteins
Cell culture
Cell Culture Techniques
Proteome
Serum
Immunoblotting
Mass spectrometry
Assays
Mass Spectrometry
Gels
Enzyme-Linked Immunosorbent Assay

Keywords

  • circulating tumor markers
  • fascin
  • head and neck cancer
  • tumor interstitial fluid

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Fascin is a circulating tumor marker for head and neck cancer as determined by a proteomic analysis of interstitial fluid from the tumor microenvironment. / Chang, Joseph Tung Chieh; Lee, Li Yu; Chen, Yin Ju; Lu, Ya Ching; Liao, Chun Ta; Chen, I. How; Huang, Yu Chen; Chen, Wen Ho; Huang, Chi Che; Tsai, Chi Ying; Cheng, Ann Joy.

In: Clinical Chemistry and Laboratory Medicine, Vol. 53, No. 10, 01.09.2015, p. 1631-1641.

Research output: Contribution to journalArticle

Chang, Joseph Tung Chieh ; Lee, Li Yu ; Chen, Yin Ju ; Lu, Ya Ching ; Liao, Chun Ta ; Chen, I. How ; Huang, Yu Chen ; Chen, Wen Ho ; Huang, Chi Che ; Tsai, Chi Ying ; Cheng, Ann Joy. / Fascin is a circulating tumor marker for head and neck cancer as determined by a proteomic analysis of interstitial fluid from the tumor microenvironment. In: Clinical Chemistry and Laboratory Medicine. 2015 ; Vol. 53, No. 10. pp. 1631-1641.
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AU - Liao, Chun Ta

AU - Chen, I. How

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AU - Tsai, Chi Ying

AU - Cheng, Ann Joy

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N2 - Background: Head and neck cancer (HNC) is a prevalent cancer worldwide; however, clinically useful tumor markers for HNC have not been identified. Here, we aimed to identify secretory proteins from the tumor microenvironment as candidate circulating tumor markers. Methods: Samples derived from seven pairs of tumor interstitial fluid (TIF) and normal interstitial fluid (NIF) samples from patients with HNC were analyzed. The proteomes were determined by gel-based-mass-spectrometry proteomic methods. The most up-regulated protein, fascin was confirmed in the cancer tissues and cell culture supernatant by immunoblotting and immunohistochemistry assays. Serum fascin was determined in 40 HNC and 40 normal individuals by ELISA. Results: After proteomics analysis, 189 peptides were identified, corresponding to 75 proteins. Of the 21 proteins which were identified more than twice, five up-regulated proteins identified most frequently including fascin. The most elevated fascin was over-expressed in cancer tissues and cell culture supernatant. Serum fascin was significantly up-regulated in the cancer patients (p

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