False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome

Kuo Chien Tsao, Guang Wu Chen, Chung Guei Huang, Ya Ling Huang, Jing Yi Lin, Mok Chee Keng, Chien Fen Sun, Shin Ru Shih

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Taiwan suffered from the outbreak of severe acute respiratory syndrome (SARS) in 2003. Our laboratory performed a series of virology and serology tests for SARS patients admitted to our hospital. Cross-reactivity was found when testing for antibody against human T-cell lymphotropic virus (HTLV) in one patient with SARS. Therefore, antibodies against HTLV were examined in paired-sera from 26 SARS patients. ELISA and a neutralization test were used to measure anti-SARS antibodies. Seroconversion for antibody against SARS-CoV was observed in all patients. Surprisingly, with the use of ELISA for HTLV, sera for 13 patients were positive for HTLV (50%), and seroconversion for HTLV was also observed in 10 patients (38.5%). Western blot for HTLV on those 26 paired-sera from 13 HTLV-positive patients displayed 5 positive results for HTLV-I, 7 positive results for HTLV-II, 1 positive result for both HTLV-I and II, 9 negative results for either HTLV-I or HTLV-II, and 4 "indeterminate" results. The findings that antibody to HTLV can be detected in blood samples collected from SARS patients provide important information for safe handling of blood products. Without such knowledge, blood products can be discarded mistakenly even though they contain anti-SARS-CoV antibodies that may be potentially valuable for SARS therapy.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalJournal of Medical Virology
Volume77
Issue number3
DOIs
Publication statusPublished - Nov 2005
Externally publishedYes

Fingerprint

Severe Acute Respiratory Syndrome
Viruses
T-Lymphocytes
Antibodies
Human T-lymphotropic virus 2
Serum
Enzyme-Linked Immunosorbent Assay
Respiratory Therapy
Neutralization Tests
Virology
Serology
Taiwan
Disease Outbreaks

Keywords

  • Cross-reactivity
  • Human T-cell lymphotropic virus (HTLV)
  • Severe acute respiratory syndrome (SARS)

ASJC Scopus subject areas

  • Virology

Cite this

Tsao, K. C., Chen, G. W., Huang, C. G., Huang, Y. L., Lin, J. Y., Keng, M. C., ... Shih, S. R. (2005). False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome. Journal of Medical Virology, 77(3), 331-336. https://doi.org/10.1002/jmv.20460

False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome. / Tsao, Kuo Chien; Chen, Guang Wu; Huang, Chung Guei; Huang, Ya Ling; Lin, Jing Yi; Keng, Mok Chee; Sun, Chien Fen; Shih, Shin Ru.

In: Journal of Medical Virology, Vol. 77, No. 3, 11.2005, p. 331-336.

Research output: Contribution to journalArticle

Tsao, Kuo Chien ; Chen, Guang Wu ; Huang, Chung Guei ; Huang, Ya Ling ; Lin, Jing Yi ; Keng, Mok Chee ; Sun, Chien Fen ; Shih, Shin Ru. / False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome. In: Journal of Medical Virology. 2005 ; Vol. 77, No. 3. pp. 331-336.
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