Facile method for determination of deoxycytidine kinase activity in biological milieus

Wei Hua Hao, Li Chieh Yang, Jong Jing Wang, Chang Shan Hsu, Li Chien Chang, Kuang Yang Hsu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

Original languageEnglish
Pages (from-to)236-241
Number of pages6
JournalJournal of Food and Drug Analysis
Volume22
Issue number2
DOIs
Publication statusPublished - Jun 1 2014

Fingerprint

Deoxycytidine Kinase
bioactive properties
phosphotransferases (kinases)
adenosine triphosphate
gemcitabine
Luminescence
Adenosine Triphosphate
methodology
cervix
luminescence
chemiluminescence
reversed-phase liquid chromatography
Reverse-Phase Chromatography
luciferase
radionuclides
Luciferases
application methods
Cervix Uteri
Radioisotopes
cultured cells

Keywords

  • Chemiluminescent assay
  • Deoxycytidine kinase
  • Gemcitabine

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Facile method for determination of deoxycytidine kinase activity in biological milieus. / Hao, Wei Hua; Yang, Li Chieh; Wang, Jong Jing; Hsu, Chang Shan; Chang, Li Chien; Hsu, Kuang Yang.

In: Journal of Food and Drug Analysis, Vol. 22, No. 2, 01.06.2014, p. 236-241.

Research output: Contribution to journalArticle

Hao, Wei Hua ; Yang, Li Chieh ; Wang, Jong Jing ; Hsu, Chang Shan ; Chang, Li Chien ; Hsu, Kuang Yang. / Facile method for determination of deoxycytidine kinase activity in biological milieus. In: Journal of Food and Drug Analysis. 2014 ; Vol. 22, No. 2. pp. 236-241.
@article{8678e5a36fdf4d0d9c3a6e54b1469f72,
title = "Facile method for determination of deoxycytidine kinase activity in biological milieus",
abstract = "A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.",
keywords = "Chemiluminescent assay, Deoxycytidine kinase, Gemcitabine",
author = "Hao, {Wei Hua} and Yang, {Li Chieh} and Wang, {Jong Jing} and Hsu, {Chang Shan} and Chang, {Li Chien} and Hsu, {Kuang Yang}",
year = "2014",
month = "6",
day = "1",
doi = "10.1016/j.jfda.2013.09.008",
language = "English",
volume = "22",
pages = "236--241",
journal = "Journal of Food and Drug Analysis",
issn = "1021-9498",
publisher = "Elsevier Taiwan LLC",
number = "2",

}

TY - JOUR

T1 - Facile method for determination of deoxycytidine kinase activity in biological milieus

AU - Hao, Wei Hua

AU - Yang, Li Chieh

AU - Wang, Jong Jing

AU - Hsu, Chang Shan

AU - Chang, Li Chien

AU - Hsu, Kuang Yang

PY - 2014/6/1

Y1 - 2014/6/1

N2 - A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

AB - A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

KW - Chemiluminescent assay

KW - Deoxycytidine kinase

KW - Gemcitabine

UR - http://www.scopus.com/inward/record.url?scp=84914678280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914678280&partnerID=8YFLogxK

U2 - 10.1016/j.jfda.2013.09.008

DO - 10.1016/j.jfda.2013.09.008

M3 - Article

AN - SCOPUS:84914678280

VL - 22

SP - 236

EP - 241

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

SN - 1021-9498

IS - 2

ER -