Fabrication of large perfusable macroporous cell-laden hydrogel scaffolds using microbial transglutaminase

Pei Yu Chen, Kai Chiang Yang, Chang Chin Wu, Jeen Huei Yu, Feng Huei Lin, Jui Sheng Sun

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In this study, we developed a method to fabricate large, perfusable, macroporous, cell-laden hydrogels. This method is suitable for efficient cell seeding, and can maintain sufficient oxygen delivery and mass transfer. We first loaded three types of testing cells (including NIH 3T3, ADSC and Huh7) into gelatin hydrogel filaments, then cross-linked the cell-laden gelatin hydrogel filaments using microbial transglutaminase (mTGase). In situ cross-linking by mTGase was found to be non-cytotoxic and prevented the scattering of the cells after delivery. The gelatin hydrogel constructs kept the carried cells viable; also, the porosity and permeability were adequate for a perfusion system. Cell proliferation was better under perfusion culture than under static culture. When human umbilical vein endothelial cells were seeded into the constructs, we demonstrated that they stably formed an even coverage on the surface of the hydrogel filaments, serving as a preliminary microvasculature network. We concluded that this method provides a viable solution for cell seeding, oxygen delivery, and mass transfer in large three-dimensional (3-D) tissue engineering. Furthermore, it has the potential for being a workhorse in studies involving 3-D cell cultures and tissue engineering.

Original languageEnglish
Pages (from-to)912-920
Number of pages9
JournalActa Biomaterialia
Volume10
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Transglutaminases
Hydrogel
Hydrogels
Scaffolds
Gelatin
Cell culture
Fabrication
Tissue engineering
Mass transfer
Tissue Engineering
Oxygen
Bioelectric potentials
Endothelial cells
Cell proliferation
Perfusion
Cell Engineering
NIH 3T3 Cells
Somatostatin-Secreting Cells
Porosity
Human Umbilical Vein Endothelial Cells

Keywords

  • Cell culture
  • Hydrogel
  • Scaffold
  • Three-dimensional
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Fabrication of large perfusable macroporous cell-laden hydrogel scaffolds using microbial transglutaminase. / Chen, Pei Yu; Yang, Kai Chiang; Wu, Chang Chin; Yu, Jeen Huei; Lin, Feng Huei; Sun, Jui Sheng.

In: Acta Biomaterialia, Vol. 10, No. 2, 2014, p. 912-920.

Research output: Contribution to journalArticle

Chen, Pei Yu ; Yang, Kai Chiang ; Wu, Chang Chin ; Yu, Jeen Huei ; Lin, Feng Huei ; Sun, Jui Sheng. / Fabrication of large perfusable macroporous cell-laden hydrogel scaffolds using microbial transglutaminase. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 2. pp. 912-920.
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