Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Tina T C Tseng; Cheng Fu Chang; Wen Chin Chan

doi:10.3390/molecules19067341

Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Tina T C Tseng, Cheng Fu Chang, Wen Chin Chan

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm^-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM^-1•cm^-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

Original language	English
Pages (from-to)	7341-7355
Number of pages	15
Journal	Molecules
Volume	19
Issue number	6
DOIs	https://doi.org/10.3390/molecules19067341
Publication status	Published - 2014

Keywords

Chitosan
Enzyme immobilization
Glutamate
Glutamate oxidase
Glutaraldehyde
Hypothalamus
Microelectrode
Micromachining
Rat

ASJC Scopus subject areas

Organic Chemistry
Medicine(all)

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10.3390/molecules19067341

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title = "Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo",

abstract = "Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.",

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AU - Tseng, Tina T C

AU - Chang, Cheng Fu

AU - Chan, Wen Chin

PY - 2014

Y1 - 2014

N2 - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

AB - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

KW - Chitosan

KW - Enzyme immobilization

KW - Glutamate

KW - Glutamate oxidase

KW - Glutaraldehyde

KW - Hypothalamus

KW - Microelectrode

KW - Micromachining

KW - Rat

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Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Abstract

Keywords

ASJC Scopus subject areas

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