Abstract
Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.
| Original language | English |
|---|---|
| Pages (from-to) | 7341-7355 |
| Number of pages | 15 |
| Journal | Molecules |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Chitosan
- Enzyme immobilization
- Glutamate
- Glutamate oxidase
- Glutaraldehyde
- Hypothalamus
- Microelectrode
- Micromachining
- Rat
ASJC Scopus subject areas
- Organic Chemistry
- Medicine(all)
Cite this
Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo. / Tseng, Tina T C; Chang, Cheng Fu; Chan, Wen Chin.
In: Molecules, Vol. 19, No. 6, 2014, p. 7341-7355.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo
AU - Tseng, Tina T C
AU - Chang, Cheng Fu
AU - Chan, Wen Chin
PY - 2014
Y1 - 2014
N2 - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.
AB - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.
KW - Chitosan
KW - Enzyme immobilization
KW - Glutamate
KW - Glutamate oxidase
KW - Glutaraldehyde
KW - Hypothalamus
KW - Microelectrode
KW - Micromachining
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=84903280908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903280908&partnerID=8YFLogxK
U2 - 10.3390/molecules19067341
DO - 10.3390/molecules19067341
M3 - Article
C2 - 24905604
AN - SCOPUS:84903280908
VL - 19
SP - 7341
EP - 7355
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 6
ER -