Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Tina T C Tseng, Cheng Fu Chang, Wen Chin Chan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

Original languageEnglish
Pages (from-to)7341-7355
Number of pages15
JournalMolecules
Volume19
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • Chitosan
  • Enzyme immobilization
  • Glutamate
  • Glutamate oxidase
  • Glutaraldehyde
  • Hypothalamus
  • Microelectrode
  • Micromachining
  • Rat

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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