Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Tina T C Tseng, Cheng Fu Chang, Wen Chin Chan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

Original languageEnglish
Pages (from-to)7341-7355
Number of pages15
JournalMolecules
Volume19
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Immobilized Enzymes
glutamates
enzymes
Glutamic Acid
Fabrication
fabrication
Monitoring
sensors
Sensors
rats
Rats
hypothalamus
Microelectrodes
oxidase
Chitosan
Glutaral
crosslinking
Platinum
immobilization
Immobilization

Keywords

  • Chitosan
  • Enzyme immobilization
  • Glutamate
  • Glutamate oxidase
  • Glutaraldehyde
  • Hypothalamus
  • Microelectrode
  • Micromachining
  • Rat

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo. / Tseng, Tina T C; Chang, Cheng Fu; Chan, Wen Chin.

In: Molecules, Vol. 19, No. 6, 2014, p. 7341-7355.

Research output: Contribution to journalArticle

Tseng, Tina T C ; Chang, Cheng Fu ; Chan, Wen Chin. / Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo. In: Molecules. 2014 ; Vol. 19, No. 6. pp. 7341-7355.
@article{8878e43ac7e04eae9ad51a2e14e1c1aa,
title = "Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo",
abstract = "Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.",
keywords = "Chitosan, Enzyme immobilization, Glutamate, Glutamate oxidase, Glutaraldehyde, Hypothalamus, Microelectrode, Micromachining, Rat",
author = "Tseng, {Tina T C} and Chang, {Cheng Fu} and Chan, {Wen Chin}",
year = "2014",
doi = "10.3390/molecules19067341",
language = "English",
volume = "19",
pages = "7341--7355",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",

}

TY - JOUR

T1 - Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

AU - Tseng, Tina T C

AU - Chang, Cheng Fu

AU - Chan, Wen Chin

PY - 2014

Y1 - 2014

N2 - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

AB - Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

KW - Chitosan

KW - Enzyme immobilization

KW - Glutamate

KW - Glutamate oxidase

KW - Glutaraldehyde

KW - Hypothalamus

KW - Microelectrode

KW - Micromachining

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=84903280908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903280908&partnerID=8YFLogxK

U2 - 10.3390/molecules19067341

DO - 10.3390/molecules19067341

M3 - Article

C2 - 24905604

AN - SCOPUS:84903280908

VL - 19

SP - 7341

EP - 7355

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 6

ER -