Fabrication of chondroitin sulfate-chitosan composite artificial extracellular matrix for stabilization of fibroblast growth factor

Fwu Long Mi, Shin Shing Shyu, Chih Kang Peng, Yu Bey Wu, Hsing Wen Sung, Pei Shan Wang, Chi Chuan Huang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The development of a novel, three-dimensional, macroporous artificial extracellular matrix (AECM) based on chondroitin sulfate (ChS)-chitosan (Chito) combination is reported. The composite AECM composed of ChS-Chito conjugated network was prepared by a homogenizing interpolyelectrolyte complex/covalent conjugation technique through co-crosslinked with N,N-(3-dimethylaminopropyl)- N′-ethyl carbodiimide (EDC) and N-hydroxysuccinimide (NHS). In contrast to EDC/NHS, two different reagents, calcium ion and glutaraldehyde, were used to react with ChS or Chito for the preparation of ChS-Chito composites containing crosslinked ChS or Chito network in the matrix. The stability and in vitro enzymatic degradability of the glutaraldehyde-, EDC/NHS-, and Ca 2+-crosslinked ChS-Chito composite AECMs were all investigated in this study. The results showed that crosslinking improved the stability of prepared ChS-Chito AECMs in physiological buffer solution (PBS) and provided superior protective effect against the enzymatic hydrolysis of ChS, compared with their non-crosslinked counterpart. Because ChS was a heparin-like glycosaminoglycan (GAG), the ChS-Chito composite AECMs appeared to promote binding efficiency for basic fibroblast growth factor (bFGF). The bFGF releasing from the ChS-Chito composite AECMs retained its biological activity as examined by the in vitro proliferation of human fibroblast, depending on the crosslinking mode for the preparation of these composite AECMs. Histological assay showed that the EDC/NHS-crosslinked ChS-Chito composite AECM, after incorporated with bFGF, was biodegradable and could result in a significantly enhanced vascularization effect and tissue penetration. These results suggest that the ChS-Chito composite AECMs fabricated in this study may be a promising approach for tissue-engineering application.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalJournal of Biomedical Materials Research - Part A
Volume76
Issue number1
DOIs
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Fibroblast Growth Factors
Chondroitin Sulfates
Chitosan
Fibroblasts
Stabilization
Fabrication
Composite materials
Fibroblast Growth Factor 2
Glutaral
Crosslinking
Intercellular Signaling Peptides and Proteins
Sulfates
Enzymatic hydrolysis
Bioactivity
Tissue engineering
Heparin
Assays
Buffers
Calcium

Keywords

  • Artificial extracellular matrix
  • Basic fibroblast growth factor
  • Chitosan
  • Chondroitin sulfate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Fabrication of chondroitin sulfate-chitosan composite artificial extracellular matrix for stabilization of fibroblast growth factor. / Mi, Fwu Long; Shyu, Shin Shing; Peng, Chih Kang; Wu, Yu Bey; Sung, Hsing Wen; Wang, Pei Shan; Huang, Chi Chuan.

In: Journal of Biomedical Materials Research - Part A, Vol. 76, No. 1, 01.2006, p. 1-15.

Research output: Contribution to journalArticle

Mi, Fwu Long ; Shyu, Shin Shing ; Peng, Chih Kang ; Wu, Yu Bey ; Sung, Hsing Wen ; Wang, Pei Shan ; Huang, Chi Chuan. / Fabrication of chondroitin sulfate-chitosan composite artificial extracellular matrix for stabilization of fibroblast growth factor. In: Journal of Biomedical Materials Research - Part A. 2006 ; Vol. 76, No. 1. pp. 1-15.
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