Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis

Yen Hsiang Chang, Zhi Meng Chen, Hsien Yu Peng, Yuan Jen Tsai, Tzer Bin Lin, Gin Den Chen, C. R. Chris Wang, Sarah Y. Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalJournal of the Chinese Chemical Society
Volume58
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Iron oxide nanoparticles
  • MALDI/MS
  • Rat spinal cord samples
  • Substance P

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis'. Together they form a unique fingerprint.

  • Cite this