Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis

Yen Hsiang Chang, Zhi Meng Chen, Hsien Yu Peng, Yuan Jen Tsai, Tzer Bin Lin, Gin Den Chen, C. R. Chris Wang, Sarah Y. Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalJournal of the Chinese Chemical Society
Volume58
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Substance P
Desorption
Nanoparticles
Lasers
Ionization
Rats
Coulomb interactions
ferric oxide
Washing
Surface-Active Agents
Buffers
Salts
Adsorption
Recovery

Keywords

  • Iron oxide nanoparticles
  • MALDI/MS
  • Rat spinal cord samples
  • Substance P

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis. / Chang, Yen Hsiang; Chen, Zhi Meng; Peng, Hsien Yu; Tsai, Yuan Jen; Lin, Tzer Bin; Chen, Gin Den; Chris Wang, C. R.; Chang, Sarah Y.

In: Journal of the Chinese Chemical Society, Vol. 58, No. 4, 2011, p. 457-462.

Research output: Contribution to journalArticle

Chang, Yen Hsiang ; Chen, Zhi Meng ; Peng, Hsien Yu ; Tsai, Yuan Jen ; Lin, Tzer Bin ; Chen, Gin Den ; Chris Wang, C. R. ; Chang, Sarah Y. / Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis. In: Journal of the Chinese Chemical Society. 2011 ; Vol. 58, No. 4. pp. 457-462.
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AU - Chen, Gin Den

AU - Chris Wang, C. R.

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