Extracorporeal shock wave promotes growth and differentiation of bone-marrow stromal cells towards osteoprogenitors associated with induction of TGF-β1

F. S. Wang, K. D. Yang, R. F. Chen, C. J. Wang, S. M. Sheen-Chen

Research output: Contribution to journalArticle

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Abstract

Extracorporeal shock-wave (ESW) treatment has been shown to be effective in promoting the healing of fractures. We aimed to determine whether ESW could enhance the growth of bone-marrow osteoprogenitor cells. We applied ESW to the left femur of rats 10 mm above the knee at 0.16 mJ/mm 2 in a range of between 250 and 2000 impulses. Bone-marrow cells were harvested after ESW for one day and subjected to assessment of colony-forming unit (CFU) granulocytes, monocytes, erythrocytes, megakaryocytes (CFU-Mix), CFU-stromal cells (CFU-S) and CFU-osteoprogenitors (CFU-O). We found that the mean value for the CFU-O colonies after treatment with 500 impulses of ESW was 168.2 CFU-O/well (SEM 11.3) compared with 88.2 CFU-O/well (SEM 7.2) in the control group. By contrast, ESW treatment did not affect haematopoiesis as shown by the CFU-Mix (p = 0.557). Treatment with 250 and 500 impulses promoted CFU-O, but not CFU-Mix formations whereas treatment with more than 750 impulses had an inhibiting effect. Treatment with 500 impulses also enhanced the activity of bone alkaline phosphatase in the subculture of CFU-O (p<0.01), indicating a selective promotion of growth of osteoprogenitor cells. Similarly, formation of bone nodules in the long-term culture of bone-marrow osteoprogenitor cells was also significantly enhanced by ESW treatment with 500 impulses. The mean production of TGF-β1 was 610 pg/ml (SEM 84.6) in culture supernatants from ESW-treated rats compared with 283 pg/ml (SEM 36.8) in the control group. Our findings suggest that optimal treatment with ESW could enhance rat bone-marrow stromal growth and differentiation towards osteoprogenitors presumably by induction of TGF-β1.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalJournal of Bone and Joint Surgery - Series B
Volume84
Issue number3
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

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Mesenchymal Stromal Cells
Stem Cells
Shock
Growth
Bone Marrow Cells
Control Groups
Fracture Healing
Megakaryocytes
Hematopoiesis
Stromal Cells
Granulocytes
Osteogenesis
Femur
Alkaline Phosphatase
Monocytes
Knee
Erythrocytes
Bone Marrow
Bone and Bones

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Extracorporeal shock wave promotes growth and differentiation of bone-marrow stromal cells towards osteoprogenitors associated with induction of TGF-β1. / Wang, F. S.; Yang, K. D.; Chen, R. F.; Wang, C. J.; Sheen-Chen, S. M.

In: Journal of Bone and Joint Surgery - Series B, Vol. 84, No. 3, 01.01.2002, p. 457-461.

Research output: Contribution to journalArticle

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