Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers.

Y. Takada, E. A. Wayner, W. G. Carter, M. E. Hemler

Research output: Contribution to journalArticle

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Abstract

The Very Late Activation Antigen (VLA) proteins are a family of five related heterodimers, which also are part of the integrin superfamily of cell adhesion molecules. Except for the identification of VLA-5 as a fibronectin receptor structure, the functions of the VLA proteins have remained unclarified. In this paper, immunoprecipitation experiments with both anti-alpha and anti-beta subunit antibodies showed that the previously identified cell adhesion receptor for collagen, extracellular matrix receptor II (ECMRII), is equivalent to VLA-2. At the same time a previously described multispecific cell adhesion receptor for collagen, fibronectin, and laminin (ECMRI) has been shown to be identical to VLA-3. Although the mAb 12F1 and P1H5 both recognized VLA-2 (ECMRII), they appeared to define distinct epitopes on the alpha 2 subunit. On the other hand, the mAb P1B5 and J143 recognized the alpha 3 subunit of VLA-3 (ECMRI) at or near the same site. Consistent with the collagen receptor functions of VLA-2 (ECMRII) and VLA-3 (ECMRI), anti-VLA beta antiserum blocked cell attachment to collagen.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalJournal of Cellular Biochemistry
Volume37
Issue number4
Publication statusPublished - Aug 1988
Externally publishedYes

Fingerprint

Integrin alpha3beta1
Integrin alpha2beta1
Fibronectins
Collagen
Chemical activation
Antigens
Cell Adhesion
Cell adhesion
Collagen Receptors
Integrin alpha5beta1
Cell Adhesion Molecules
Laminin
extracellular matrix receptor
Immunoprecipitation
Integrins
Immune Sera
Epitopes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers. / Takada, Y.; Wayner, E. A.; Carter, W. G.; Hemler, M. E.

In: Journal of Cellular Biochemistry, Vol. 37, No. 4, 08.1988, p. 385-393.

Research output: Contribution to journalArticle

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abstract = "The Very Late Activation Antigen (VLA) proteins are a family of five related heterodimers, which also are part of the integrin superfamily of cell adhesion molecules. Except for the identification of VLA-5 as a fibronectin receptor structure, the functions of the VLA proteins have remained unclarified. In this paper, immunoprecipitation experiments with both anti-alpha and anti-beta subunit antibodies showed that the previously identified cell adhesion receptor for collagen, extracellular matrix receptor II (ECMRII), is equivalent to VLA-2. At the same time a previously described multispecific cell adhesion receptor for collagen, fibronectin, and laminin (ECMRI) has been shown to be identical to VLA-3. Although the mAb 12F1 and P1H5 both recognized VLA-2 (ECMRII), they appeared to define distinct epitopes on the alpha 2 subunit. On the other hand, the mAb P1B5 and J143 recognized the alpha 3 subunit of VLA-3 (ECMRI) at or near the same site. Consistent with the collagen receptor functions of VLA-2 (ECMRII) and VLA-3 (ECMRI), anti-VLA beta antiserum blocked cell attachment to collagen.",
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