Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA‐2 and VLA‐3 in the VLA family of heterodimers

Yoshikazu Takada, Elizabeth A. Wayner, William G. Carter, Martin E. Hemler

Research output: Contribution to journalArticlepeer-review

134 Citations (Scopus)

Abstract

The Very Late Activation Antigen (VLA) proteins are a family of five related heterodimers, which also are part of the integrin superfamily of cell adhesion molecules. Except for the identification of VLA‐5 as a fibronectin receptor structure, the functions of the VLA proteins have remained unclarified. In this paper, immuno‐precipitation experiments with both anti‐α and anti‐β subunit antibodies showed that the previously identified cell adhesion receptor for collagen, extracellular matrix receptor II (ECMRII), is equivalent to VLA‐2. At the same time a previously described multispecific cell adhesion receptor for collagen, fibroncclin, and laminin (ECMRI) has been shown to be identical to VLA‐3. Although the mAb 12F1 and P1H5 both recognized VLA‐2 (ECMRII), they appeared to define distinct epitopes on the α2 subunit. On the other hand, the mAb PIB5 and J143 recognized the α3 subunit of VLA‐3 (ECMRI) at or near the same site. Consistent with the collagen receptor functions of VLA‐2 (ECMRII) and VLA‐3 (ECMRI), anti‐VLA β antiserum blocked cell attachment to collagen.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalJournal of Cellular Biochemistry
Volume37
Issue number4
DOIs
Publication statusPublished - Aug 1988

Keywords

  • VLA proteins
  • arg‐gly‐asp amino acid sequence
  • cell adhesion
  • integrin superfamily

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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