Extracellular matrix of collagen modulates intracellular calcium handling and electrophysiological characteristics of HL-1 cardiomyocytes with activation of angiotensin II type 1 receptor

Yen Yu Lu, Yao Chang Chen, Yu Hsun Kao, Tsu Juey Wu, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Myocardial fibrosis plays a critical role in heart failure, resulting in cardiac structural and electrical remodeling which can induce atrial arrhythmias. Collagen is the major element of fibrosis. However, it is not clear whether collagen can directly regulate the calcium homeostasis and the electrophysiologic characteristics of cardiomyocytes. The aim of this study was to determine the effects of collagen on calcium homeostasis and the electrical properties of atrial cardiomyocytes. Methods and Results: HL-1 cardiomyocytes were cultured with and without collagen type I (1 or 10 μg/mL) or losartan (10 μmol/L). Whole-cell clamp, indo-1 fluorescence, and Western blotting were used to evaluate the action potential (AP) and ionic currents, intracellular calcium homeostasis, and calcium regulatory proteins. Compared with the control samples, there was no significant difference in collagen (1 μg/mL)-treated HL-1 cardiomyocytes. However, collagen (10 μg/mL)-treated HL-1 cardiomyocytes exhibited larger intracellular calcium ([Ca2+]i) transients by 113% and a larger sarcoplasmic reticulum calcium content by 86%. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes had higher expression of sarcoplasmic reticulum ATPase (SERCA2a) and Thr17-phosphorylated phospholamban but similar protein expressions of the Na+/Ca2+ exchanger and ryanodine receptor. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes (n = 11) had larger AP amplitude (104 ± 5 vs 83 ± 7 mV; P <.05), and shorter 90% of AP duration (25 ± 2 vs 33 ± 2 ms, P <.05) than control cells (n = 11). Moreover, collagen (10 μg/mL)-treated HL-1 cells had larger Ito and IKsus values than control cells. The administration of losartan (10 μmol/L) attenuated collagen-induced changes in [Ca2+]i transients, [Ca2+]i stores, AP morphology, ionic currents, SERCA2a, and Thr17-phosphorylated phospholamban expressions. Conclusions: This study demonstrates that collagen can directly modulate the calcium dynamics and electrical activities of atrial cardiomyocytes, which are associated with the renin-angiotensin system. These findings suggest a critical role of collagen in electrical remodeling during fibrosis.

Original languageEnglish
Pages (from-to)82-94
Number of pages13
JournalJournal of Cardiac Failure
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 2011

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Angiotensin Type 1 Receptor
Cardiac Myocytes
Extracellular Matrix
Collagen
Calcium
Action Potentials
Atrial Remodeling
Homeostasis
Fibrosis
Losartan
Sarcoplasmic Reticulum
Collagen Type X
Ryanodine Receptor Calcium Release Channel
Renin-Angiotensin System
Collagen Type I
Adenosine Triphosphatases
Cardiac Arrhythmias
Proteins
Heart Failure
Fluorescence

Keywords

  • calcium homeostasis
  • electrophysiology
  • extracellular matrix
  • Fibrosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

@article{5b9efb512a6e47e99645c7c67e48cebf,
title = "Extracellular matrix of collagen modulates intracellular calcium handling and electrophysiological characteristics of HL-1 cardiomyocytes with activation of angiotensin II type 1 receptor",
abstract = "Background: Myocardial fibrosis plays a critical role in heart failure, resulting in cardiac structural and electrical remodeling which can induce atrial arrhythmias. Collagen is the major element of fibrosis. However, it is not clear whether collagen can directly regulate the calcium homeostasis and the electrophysiologic characteristics of cardiomyocytes. The aim of this study was to determine the effects of collagen on calcium homeostasis and the electrical properties of atrial cardiomyocytes. Methods and Results: HL-1 cardiomyocytes were cultured with and without collagen type I (1 or 10 μg/mL) or losartan (10 μmol/L). Whole-cell clamp, indo-1 fluorescence, and Western blotting were used to evaluate the action potential (AP) and ionic currents, intracellular calcium homeostasis, and calcium regulatory proteins. Compared with the control samples, there was no significant difference in collagen (1 μg/mL)-treated HL-1 cardiomyocytes. However, collagen (10 μg/mL)-treated HL-1 cardiomyocytes exhibited larger intracellular calcium ([Ca2+]i) transients by 113{\%} and a larger sarcoplasmic reticulum calcium content by 86{\%}. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes had higher expression of sarcoplasmic reticulum ATPase (SERCA2a) and Thr17-phosphorylated phospholamban but similar protein expressions of the Na+/Ca2+ exchanger and ryanodine receptor. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes (n = 11) had larger AP amplitude (104 ± 5 vs 83 ± 7 mV; P <.05), and shorter 90{\%} of AP duration (25 ± 2 vs 33 ± 2 ms, P <.05) than control cells (n = 11). Moreover, collagen (10 μg/mL)-treated HL-1 cells had larger Ito and IKsus values than control cells. The administration of losartan (10 μmol/L) attenuated collagen-induced changes in [Ca2+]i transients, [Ca2+]i stores, AP morphology, ionic currents, SERCA2a, and Thr17-phosphorylated phospholamban expressions. Conclusions: This study demonstrates that collagen can directly modulate the calcium dynamics and electrical activities of atrial cardiomyocytes, which are associated with the renin-angiotensin system. These findings suggest a critical role of collagen in electrical remodeling during fibrosis.",
keywords = "calcium homeostasis, electrophysiology, extracellular matrix, Fibrosis",
author = "Lu, {Yen Yu} and Chen, {Yao Chang} and Kao, {Yu Hsun} and Wu, {Tsu Juey} and Chen, {Shih Ann} and Chen, {Yi Jen}",
year = "2011",
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language = "English",
volume = "17",
pages = "82--94",
journal = "Journal of Cardiac Failure",
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TY - JOUR

T1 - Extracellular matrix of collagen modulates intracellular calcium handling and electrophysiological characteristics of HL-1 cardiomyocytes with activation of angiotensin II type 1 receptor

AU - Lu, Yen Yu

AU - Chen, Yao Chang

AU - Kao, Yu Hsun

AU - Wu, Tsu Juey

AU - Chen, Shih Ann

AU - Chen, Yi Jen

PY - 2011/1

Y1 - 2011/1

N2 - Background: Myocardial fibrosis plays a critical role in heart failure, resulting in cardiac structural and electrical remodeling which can induce atrial arrhythmias. Collagen is the major element of fibrosis. However, it is not clear whether collagen can directly regulate the calcium homeostasis and the electrophysiologic characteristics of cardiomyocytes. The aim of this study was to determine the effects of collagen on calcium homeostasis and the electrical properties of atrial cardiomyocytes. Methods and Results: HL-1 cardiomyocytes were cultured with and without collagen type I (1 or 10 μg/mL) or losartan (10 μmol/L). Whole-cell clamp, indo-1 fluorescence, and Western blotting were used to evaluate the action potential (AP) and ionic currents, intracellular calcium homeostasis, and calcium regulatory proteins. Compared with the control samples, there was no significant difference in collagen (1 μg/mL)-treated HL-1 cardiomyocytes. However, collagen (10 μg/mL)-treated HL-1 cardiomyocytes exhibited larger intracellular calcium ([Ca2+]i) transients by 113% and a larger sarcoplasmic reticulum calcium content by 86%. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes had higher expression of sarcoplasmic reticulum ATPase (SERCA2a) and Thr17-phosphorylated phospholamban but similar protein expressions of the Na+/Ca2+ exchanger and ryanodine receptor. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes (n = 11) had larger AP amplitude (104 ± 5 vs 83 ± 7 mV; P <.05), and shorter 90% of AP duration (25 ± 2 vs 33 ± 2 ms, P <.05) than control cells (n = 11). Moreover, collagen (10 μg/mL)-treated HL-1 cells had larger Ito and IKsus values than control cells. The administration of losartan (10 μmol/L) attenuated collagen-induced changes in [Ca2+]i transients, [Ca2+]i stores, AP morphology, ionic currents, SERCA2a, and Thr17-phosphorylated phospholamban expressions. Conclusions: This study demonstrates that collagen can directly modulate the calcium dynamics and electrical activities of atrial cardiomyocytes, which are associated with the renin-angiotensin system. These findings suggest a critical role of collagen in electrical remodeling during fibrosis.

AB - Background: Myocardial fibrosis plays a critical role in heart failure, resulting in cardiac structural and electrical remodeling which can induce atrial arrhythmias. Collagen is the major element of fibrosis. However, it is not clear whether collagen can directly regulate the calcium homeostasis and the electrophysiologic characteristics of cardiomyocytes. The aim of this study was to determine the effects of collagen on calcium homeostasis and the electrical properties of atrial cardiomyocytes. Methods and Results: HL-1 cardiomyocytes were cultured with and without collagen type I (1 or 10 μg/mL) or losartan (10 μmol/L). Whole-cell clamp, indo-1 fluorescence, and Western blotting were used to evaluate the action potential (AP) and ionic currents, intracellular calcium homeostasis, and calcium regulatory proteins. Compared with the control samples, there was no significant difference in collagen (1 μg/mL)-treated HL-1 cardiomyocytes. However, collagen (10 μg/mL)-treated HL-1 cardiomyocytes exhibited larger intracellular calcium ([Ca2+]i) transients by 113% and a larger sarcoplasmic reticulum calcium content by 86%. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes had higher expression of sarcoplasmic reticulum ATPase (SERCA2a) and Thr17-phosphorylated phospholamban but similar protein expressions of the Na+/Ca2+ exchanger and ryanodine receptor. Collagen (10 μg/mL)-treated HL-1 cardiomyocytes (n = 11) had larger AP amplitude (104 ± 5 vs 83 ± 7 mV; P <.05), and shorter 90% of AP duration (25 ± 2 vs 33 ± 2 ms, P <.05) than control cells (n = 11). Moreover, collagen (10 μg/mL)-treated HL-1 cells had larger Ito and IKsus values than control cells. The administration of losartan (10 μmol/L) attenuated collagen-induced changes in [Ca2+]i transients, [Ca2+]i stores, AP morphology, ionic currents, SERCA2a, and Thr17-phosphorylated phospholamban expressions. Conclusions: This study demonstrates that collagen can directly modulate the calcium dynamics and electrical activities of atrial cardiomyocytes, which are associated with the renin-angiotensin system. These findings suggest a critical role of collagen in electrical remodeling during fibrosis.

KW - calcium homeostasis

KW - electrophysiology

KW - extracellular matrix

KW - Fibrosis

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