External urethral sphincter activity in a rat model of pudendal nerve injury

Chih Wei Peng, Jia Jin Jason Chen, Hui Yi Chang, William C. De Groat, Chen Li Cheng

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Aims: Pudendal nerve injury in the rat has been a useful animal model for studying stress urinary incontinence (SUI). However, the effect of pudendal nerve injury on activity of the external urethral sphincter (EUS) is relatively unexplored. The aims of this study were to examine voiding and the EUS electromyogram (EMG) in a durable SUI model in rats with bilateral or unilateral pudendal nerve transections. In addition, the effects of denervation on urethral anatomy were investigated. Methods: A leak point pressure (LPP) test was first used to demonstrate that pudendal nerve transection induced SUI. Cystometry exhibited changes in voiding function and EUS-EMG measurements provided a quantitative evaluation of EUS activity during voiding. The morphological changes in sections through the mid-urethra were assessed with hematoxylin and eosin (H&E) staining. Results: A significant decrease in average LPP was detected in rats 6 weeks after bilateral pudendal nerve transection (BPNT). Abnormal urodynamic measurements including a decrease in contraction amplitude and voided volume as well as an increase in contraction duration, and residual volume all indicated inefficient voiding. In addition EUS-EMG silent periods were reduced and the frequency of EUS-EMG bursting during voiding was increased. Atrophy of striated muscle in the EUS was also detected in rats with pudendal nerve transection(s). Conclusions: Our results indicate that pudendal nerve transection in rats decreases urethral outlet resistance and causes striated muscle atrophy in the EUS, EUS-EMG abnormalities and inefficient voiding. The results demonstrate that BPNT is a durable model for SUI.

Original languageEnglish
Pages (from-to)388-396
Number of pages9
JournalNeurourology and Urodynamics
Volume25
Issue number4
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Pudendal Nerve
Urethra
Wounds and Injuries
Electromyography
Stress Urinary Incontinence
Striated Muscle
Pressure
Residual Volume
Muscular Atrophy
Urodynamics
Denervation
Hematoxylin
Eosine Yellowish-(YS)
Atrophy
Anatomy
Animal Models
Staining and Labeling

Keywords

  • Cystometrographic
  • Electromyogram
  • Leak point pressure
  • Stress urinary incontinence
  • Voiding

ASJC Scopus subject areas

  • Clinical Neurology
  • Nephrology
  • Urology

Cite this

External urethral sphincter activity in a rat model of pudendal nerve injury. / Peng, Chih Wei; Chen, Jia Jin Jason; Chang, Hui Yi; De Groat, William C.; Cheng, Chen Li.

In: Neurourology and Urodynamics, Vol. 25, No. 4, 2006, p. 388-396.

Research output: Contribution to journalArticle

Peng, Chih Wei ; Chen, Jia Jin Jason ; Chang, Hui Yi ; De Groat, William C. ; Cheng, Chen Li. / External urethral sphincter activity in a rat model of pudendal nerve injury. In: Neurourology and Urodynamics. 2006 ; Vol. 25, No. 4. pp. 388-396.
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