Expression spectra of matrix metalloproteinases in metastatic non-small cell lung cancer

Torng Sen Lin, Shiow Her Chiou, Liang Shun Wang, Hsuan Hua Huang, Shu Fen Chiang, Alex Y H Shih, Y. A Lin Chen, Chih Y I Chen, Chung Ping Hsu, Nan Yung Hsu, Ming Chih Chou, Shou Jen Kuo, Kuan Chih Chow

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58 Citations (Scopus)

Abstract

By combining suppression subtractive hybridization and microarray to examine gene expressions between metastatic and non-metastatic non-small cell lung cancer (NSCLC), we have identified differential expression spectra of matrix metalloproteinases (MMP). Among MMPs, expressions of MMP-13, -14, -15 and -24 decreased, those of MMP-9, -11, -12, -16, -17, -19 and -23B did not change, and those of MMP-1, -2, -7, -8 and -10 increased dramatically. Overexpressions of MMP-1, -2, -7 and -10 were confirmed by reverse transcription-polymerase chain reaction. In this study we further assessed the clinical significance of MMP-1, -2, -7 and -10. Specimens from 472 patients with completely resected NSCLC were examined by immunohistochemistry. The median follow-up period was 38 months (range, 2-113 months). Overexpression of MMP-1 was observed in 72.9% (n=344) of 472 patients, that of MMP-2 was 77.9% (n=352), MMP-7 63.3% (n=299) and that of MMP-10 was 27.1% (n=128). For patients with lymph node metastasis, MMP-1 and -2 overexpressions were not only independent prognostic factors for unfavorable outcome, but also associated with decreased survival (p=0.0015, and p=0.011 respectively). The present study showed that MMP expression spectrum in NSCLC was heterogeneous: expression of some MMP increased, some unchanged, while some decreased. Therefore, it should be worth determining MMP expression pattern as a regimen reference for NSCLC patients who were scheduled to receive MMP inhibitor, which was class-specific, as adjuvant therapeutic agent.

Original languageEnglish
Pages (from-to)717-723
Number of pages7
JournalOncology Reports
Volume12
Issue number4
Publication statusPublished - Oct 2004
Externally publishedYes

Keywords

  • Immunohistochemistry
  • Microarray
  • Prognosis
  • RT-PCR
  • Suppression subtractive hybridization

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Lin, T. S., Chiou, S. H., Wang, L. S., Huang, H. H., Chiang, S. F., Shih, A. Y. H., Chen, Y. A. L., Chen, C. Y. I., Hsu, C. P., Hsu, N. Y., Chou, M. C., Kuo, S. J., & Chow, K. C. (2004). Expression spectra of matrix metalloproteinases in metastatic non-small cell lung cancer. Oncology Reports, 12(4), 717-723.