Expression of the Na-K-2Cl Cotransporter in Branchial MitochondrionRich Cells of Mozambique Tilapia (Oreochromis mossambicus) Subjected to Varying Chloride Conditions

Jiun-Lin Horng, Li Yih Lin

Research output: Contribution to journalArticle

Abstract

The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl- transport through the basolateral membranes of mitochondrion-rich (MR) cells in seawater teleosts. Recently, the NKCC was also found in apical membranes of MR cells of freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze the expression levels of the NKCC in
FW-acclimated tilapia in response to different ambient chloride levels. The amount of branchial NKCC from lowCl-
- (0.002-0.006 mM)-acclimated tilapia was about 3- and 5.5-fold higher than that from normal- (0.49-0.51 mM)
and high-Cl- (9.8-10.2 mM)-acclimated tilapia. With confocal microscopy, the NKCC was localized mainly in the apical membranes of MR cells with Cl-dependent abundances. The staining intensity of the NKCC in individual MR cells was significantly higher in the low-Cl- group than in the normal and high-Cl- groups. Results suggest that the apically localized NKCC is induced by a Cl- deficiency and is probably also involved in the Cl- uptake mechanism of MR cells.
Original languageEnglish
Pages (from-to)733-740
JournalZoological Studies
Volume47
Issue number6
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Oreochromis mossambicus
Tilapia (Cichlidae)
chlorides
mitochondria
cells
active transport
immunocytochemistry
immunoblotting
seawater
uptake mechanisms

Keywords

  • Mitochondrion-rich cells
  • Tilapia (Oreochromis mossambicus)
  • Osmoregulation

Cite this

@article{6b7d3a99ad0c40efb21ed86c632cf2a6,
title = "Expression of the Na-K-2Cl Cotransporter in Branchial MitochondrionRich Cells of Mozambique Tilapia (Oreochromis mossambicus) Subjected to Varying Chloride Conditions",
abstract = "The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl- transport through the basolateral membranes of mitochondrion-rich (MR) cells in seawater teleosts. Recently, the NKCC was also found in apical membranes of MR cells of freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze the expression levels of the NKCC inFW-acclimated tilapia in response to different ambient chloride levels. The amount of branchial NKCC from lowCl-- (0.002-0.006 mM)-acclimated tilapia was about 3- and 5.5-fold higher than that from normal- (0.49-0.51 mM)and high-Cl- (9.8-10.2 mM)-acclimated tilapia. With confocal microscopy, the NKCC was localized mainly in the apical membranes of MR cells with Cl-dependent abundances. The staining intensity of the NKCC in individual MR cells was significantly higher in the low-Cl- group than in the normal and high-Cl- groups. Results suggest that the apically localized NKCC is induced by a Cl- deficiency and is probably also involved in the Cl- uptake mechanism of MR cells.",
keywords = "Mitochondrion-rich cells, Tilapia (Oreochromis mossambicus), Osmoregulation",
author = "Jiun-Lin Horng and Lin, {Li Yih}",
year = "2008",
language = "English",
volume = "47",
pages = "733--740",
journal = "Zoological Studies",
issn = "1021-5506",
publisher = "Biodiversity Research Center, Academia Sinica",
number = "6",

}

TY - JOUR

T1 - Expression of the Na-K-2Cl Cotransporter in Branchial MitochondrionRich Cells of Mozambique Tilapia (Oreochromis mossambicus) Subjected to Varying Chloride Conditions

AU - Horng, Jiun-Lin

AU - Lin, Li Yih

PY - 2008

Y1 - 2008

N2 - The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl- transport through the basolateral membranes of mitochondrion-rich (MR) cells in seawater teleosts. Recently, the NKCC was also found in apical membranes of MR cells of freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze the expression levels of the NKCC inFW-acclimated tilapia in response to different ambient chloride levels. The amount of branchial NKCC from lowCl-- (0.002-0.006 mM)-acclimated tilapia was about 3- and 5.5-fold higher than that from normal- (0.49-0.51 mM)and high-Cl- (9.8-10.2 mM)-acclimated tilapia. With confocal microscopy, the NKCC was localized mainly in the apical membranes of MR cells with Cl-dependent abundances. The staining intensity of the NKCC in individual MR cells was significantly higher in the low-Cl- group than in the normal and high-Cl- groups. Results suggest that the apically localized NKCC is induced by a Cl- deficiency and is probably also involved in the Cl- uptake mechanism of MR cells.

AB - The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl- transport through the basolateral membranes of mitochondrion-rich (MR) cells in seawater teleosts. Recently, the NKCC was also found in apical membranes of MR cells of freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze the expression levels of the NKCC inFW-acclimated tilapia in response to different ambient chloride levels. The amount of branchial NKCC from lowCl-- (0.002-0.006 mM)-acclimated tilapia was about 3- and 5.5-fold higher than that from normal- (0.49-0.51 mM)and high-Cl- (9.8-10.2 mM)-acclimated tilapia. With confocal microscopy, the NKCC was localized mainly in the apical membranes of MR cells with Cl-dependent abundances. The staining intensity of the NKCC in individual MR cells was significantly higher in the low-Cl- group than in the normal and high-Cl- groups. Results suggest that the apically localized NKCC is induced by a Cl- deficiency and is probably also involved in the Cl- uptake mechanism of MR cells.

KW - Mitochondrion-rich cells

KW - Tilapia (Oreochromis mossambicus)

KW - Osmoregulation

M3 - Article

VL - 47

SP - 733

EP - 740

JO - Zoological Studies

JF - Zoological Studies

SN - 1021-5506

IS - 6

ER -