Expression of the Epstein-Barr virus BHRF1 gene, a homologue of Bcl-2, in nasopharyngeal carcinoma tissue

Mei Ying Liu, Ya Yi Shih, Long Yuan Li, Sheng Ping Chou, Tzung Shiahn Sheen, Chi Long Chen, Czau Siung Yang, Jen Yang Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) infection is associated closely with the pathogenesis of nasopharyngeal carcinoma (NPC). The EBV gene product, BHRF1, has been demonstrated in vitro and is structurally and functionally similar to the oncogene bcl-2, that is able to protect cells from programmed cell death. To determine whether the BHRF1 gene is expressed in vivo, BHRF1 mRNA or protein were sought in tissues from NPC and non-NPC patients. BHRF1 transcripts were specifically detected in the NPC tumours (32 out of 44, 72.7%) rather than the non-NPC tissues (0 out of 25) by reverse transcription, polymerase chain reaction and Southern hybridization. Other EBV genes, such as the lytic gene BZLF1 and latent genes EBNA1 and LMP2A, were also investigated. BZLF1 transcripts also were found specifically in NPC tumours (33 out of 44, 75%). EBNA1 was expressed in 79.5% of NPC, and 28% of non- NPC, tissues and LMP2A was expressed in 70.5% of NPC, and 88% of non- NPC, tissues. BHRF1 protein was detected by immunohistochemistry in 4 metastatic NPC, of 36 NPC tissue sections available. The BHRF1 protein was distributed in both the nucleus and cytoplasm of the neoplastic epithelial cells. IgG antibody against the BHRF1 protein was detected in 6 of 17 (35.3%) NPC plasma, but the protein and IgG were both absent from the non-NPC controls. BHRF1 DNA sequences were determined for 11 NPC and 3 non-NPC samples. No sequence was specific for the EBV isolates from NPC tissue. Amine acids 79 and 88 always appeared in the same form, however, for every tested isolate and both were valine or leucine. This particular characteristic was not present in the B95-8 strain or in the corresponding regions of homologues, Bcl-2 and Bcl-XL, and was regarded as unique to Oriental EBV strains. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)241-250
Number of pages10
JournalJournal of Medical Virology
Volume61
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Human Herpesvirus 4
Genes
Carcinoma
Nasopharyngeal carcinoma
Proteins
Immunoglobulin G
Epstein-Barr Virus Infections
Valine
Oncogenes
Leucine
Reverse Transcription
Amines
Blood Proteins
Neoplasms
Cytoplasm
Cell Death
Epithelial Cells
Immunohistochemistry

Keywords

  • Bcl
  • BHRF1 expression
  • Epstein-Barr virus
  • Nasopharyngeal carcinoma

ASJC Scopus subject areas

  • Virology

Cite this

Expression of the Epstein-Barr virus BHRF1 gene, a homologue of Bcl-2, in nasopharyngeal carcinoma tissue. / Liu, Mei Ying; Shih, Ya Yi; Li, Long Yuan; Chou, Sheng Ping; Sheen, Tzung Shiahn; Chen, Chi Long; Yang, Czau Siung; Chen, Jen Yang.

In: Journal of Medical Virology, Vol. 61, No. 2, 2000, p. 241-250.

Research output: Contribution to journalArticle

Liu, Mei Ying ; Shih, Ya Yi ; Li, Long Yuan ; Chou, Sheng Ping ; Sheen, Tzung Shiahn ; Chen, Chi Long ; Yang, Czau Siung ; Chen, Jen Yang. / Expression of the Epstein-Barr virus BHRF1 gene, a homologue of Bcl-2, in nasopharyngeal carcinoma tissue. In: Journal of Medical Virology. 2000 ; Vol. 61, No. 2. pp. 241-250.
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abstract = "Epstein-Barr virus (EBV) infection is associated closely with the pathogenesis of nasopharyngeal carcinoma (NPC). The EBV gene product, BHRF1, has been demonstrated in vitro and is structurally and functionally similar to the oncogene bcl-2, that is able to protect cells from programmed cell death. To determine whether the BHRF1 gene is expressed in vivo, BHRF1 mRNA or protein were sought in tissues from NPC and non-NPC patients. BHRF1 transcripts were specifically detected in the NPC tumours (32 out of 44, 72.7{\%}) rather than the non-NPC tissues (0 out of 25) by reverse transcription, polymerase chain reaction and Southern hybridization. Other EBV genes, such as the lytic gene BZLF1 and latent genes EBNA1 and LMP2A, were also investigated. BZLF1 transcripts also were found specifically in NPC tumours (33 out of 44, 75{\%}). EBNA1 was expressed in 79.5{\%} of NPC, and 28{\%} of non- NPC, tissues and LMP2A was expressed in 70.5{\%} of NPC, and 88{\%} of non- NPC, tissues. BHRF1 protein was detected by immunohistochemistry in 4 metastatic NPC, of 36 NPC tissue sections available. The BHRF1 protein was distributed in both the nucleus and cytoplasm of the neoplastic epithelial cells. IgG antibody against the BHRF1 protein was detected in 6 of 17 (35.3{\%}) NPC plasma, but the protein and IgG were both absent from the non-NPC controls. BHRF1 DNA sequences were determined for 11 NPC and 3 non-NPC samples. No sequence was specific for the EBV isolates from NPC tissue. Amine acids 79 and 88 always appeared in the same form, however, for every tested isolate and both were valine or leucine. This particular characteristic was not present in the B95-8 strain or in the corresponding regions of homologues, Bcl-2 and Bcl-XL, and was regarded as unique to Oriental EBV strains. (C) 2000 Wiley-Liss, Inc.",
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