Expression of syndecan-1 (CD138) in nasopharyngeal carcinoma is correlated with advanced stage and poor prognosis

Chi Long Chen, Da Liang Ou

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nasopharyngeal carcinoma (NPC) is an important Epstein-Barr virus-associated head and neck malignancy in Taiwan. Syndecan-1 (CD138) is involved in growth, differentiation, invasiveness, and metastatic potential of certain tumors, but its expression in NPC has never been studied. In this study, detection of expression of syndecan-1 protein and Epstein-Barr virus-encoded latent membrane protein-1 (LMP-1) in primary, recurrent, and metastatic NPC specimens in paraffin sections was performed by immunohistochemistry. The quantity of syndecan-1 messenger RNA in tumor cells was investigated by real-time reverse transcriptase polymerase chain reaction using laser capture microdissection. The results of immunohistochemical staining of syndecan-1 and LMP-1 correlated with clinicopathologic features of NPC. Eighteen (20.9%) of 86 primary, 9 (24.3%) of 37 recurrent, and 15 (44.1%) of 34 metastatic NPC samples were positive for syndecan-1, and 37 (43.0%) primary, 18 (48.6%) recurrent, and 12 (35.3%) metastatic samples were positive for LMP-1 expression. Primary NPCs with syndecan-1 protein expression were more frequently associated with advanced clinical stages and worse 5-year survival rates than those without (P = .015 and P = .0021, respectively). Conversely, the LMP-1 expression did not correlate with tumor stage or prognosis but occurred more often in nonkeratinizing carcinoma than keratinizing squamous cell carcinoma (unpublished observation). The inverse expression of syndecan-1 and LMP-1 was noted in primary NPC specimens (total 4/18 versus 35/68, P = .05). The reverse transcriptase polymerase chain reaction revealed low syndecan-1 messenger RNA levels in both primary and metastatic NPC. In conclusion, the protein expression of syndecan-1 in 21% of primary NPC was associated with advanced disease and poor prognosis, and the protein expression correlated with transcription levels.

Original languageEnglish
Pages (from-to)1279-1285
Number of pages7
JournalHuman Pathology
Volume37
Issue number10
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Syndecan-1
Membrane Proteins
Reverse Transcriptase Polymerase Chain Reaction
Neoplasms
Proteins
Laser Capture Microdissection
Nasopharyngeal carcinoma
Messenger RNA
Human Herpesvirus 4
Taiwan
Paraffin
Real-Time Polymerase Chain Reaction
Squamous Cell Carcinoma
Neck
Immunohistochemistry
Head
Observation
Staining and Labeling
Carcinoma

Keywords

  • Epstein-Barr virus
  • Nasopharyngeal carcinoma
  • Syndecan-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of syndecan-1 (CD138) in nasopharyngeal carcinoma is correlated with advanced stage and poor prognosis. / Chen, Chi Long; Ou, Da Liang.

In: Human Pathology, Vol. 37, No. 10, 10.2006, p. 1279-1285.

Research output: Contribution to journalArticle

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abstract = "Nasopharyngeal carcinoma (NPC) is an important Epstein-Barr virus-associated head and neck malignancy in Taiwan. Syndecan-1 (CD138) is involved in growth, differentiation, invasiveness, and metastatic potential of certain tumors, but its expression in NPC has never been studied. In this study, detection of expression of syndecan-1 protein and Epstein-Barr virus-encoded latent membrane protein-1 (LMP-1) in primary, recurrent, and metastatic NPC specimens in paraffin sections was performed by immunohistochemistry. The quantity of syndecan-1 messenger RNA in tumor cells was investigated by real-time reverse transcriptase polymerase chain reaction using laser capture microdissection. The results of immunohistochemical staining of syndecan-1 and LMP-1 correlated with clinicopathologic features of NPC. Eighteen (20.9{\%}) of 86 primary, 9 (24.3{\%}) of 37 recurrent, and 15 (44.1{\%}) of 34 metastatic NPC samples were positive for syndecan-1, and 37 (43.0{\%}) primary, 18 (48.6{\%}) recurrent, and 12 (35.3{\%}) metastatic samples were positive for LMP-1 expression. Primary NPCs with syndecan-1 protein expression were more frequently associated with advanced clinical stages and worse 5-year survival rates than those without (P = .015 and P = .0021, respectively). Conversely, the LMP-1 expression did not correlate with tumor stage or prognosis but occurred more often in nonkeratinizing carcinoma than keratinizing squamous cell carcinoma (unpublished observation). The inverse expression of syndecan-1 and LMP-1 was noted in primary NPC specimens (total 4/18 versus 35/68, P = .05). The reverse transcriptase polymerase chain reaction revealed low syndecan-1 messenger RNA levels in both primary and metastatic NPC. In conclusion, the protein expression of syndecan-1 in 21{\%} of primary NPC was associated with advanced disease and poor prognosis, and the protein expression correlated with transcription levels.",
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