Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule

Yoshikatsu Kanai, Matthias G. Stelzner, Wen Sen Lee, Rebecca G. Wells, Dennis Brown, Matthias A. Hediger

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

A rat kidney- and intestine-specific cDNA (D2) that induces high-affinity, Na+-independent uptake of cystine and dibasic and neutral amino acids into cRNA-injected Xenopus oocytes was recently isolated by expression cloning in our laboratory (R. G. Wells and M. A. Hediger. Proc. Natl. Acad. Sci. USA 89: 5596-5600, 1992). At present it is not known whether the D2-encoded protein functions as a transporter or as a transporter activator. To gain more insight into the role of D2 in renal amino acid transport, we studied the site of its expression in the kidney. This was determined by Northern blot analysis and by using a combination of in situ hybridization and immunocytochemistry with antibodies that recognize specific proximal tubule segments. D2 antisense RNA hybridized to the same tubular segments that were strongly positive for anti-ecto-adenosinetriphosphatase but negative for carbonic anhydrase type IV and the facilitated glucose transporter GLUT2. We conclude that D2 mRNA is strongly expressed in the rat kidney proximal tubule S3 segment, although there is weak hybridization to the S1 and S2 segments. The signal is absent in all other parts of the kidney. The S3 specific expression of D2 mRNA coincides with the site of high-affinity transport of cystine and other amino acids, consistent with the proposed involvement of D2 in these processes.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume263
Issue number6 32-6
Publication statusPublished - Dec 1992
Externally publishedYes

Fingerprint

Kidney
Amino Acids
Messenger RNA
Cystine
Carbonic Anhydrase IV
Proteins
Diamino Amino Acids
Neutral Amino Acids
Antisense RNA
Complementary RNA
Proximal Kidney Tubule
Facilitative Glucose Transport Proteins
Xenopus
Northern Blotting
Oocytes
Intestines
In Situ Hybridization
Adenosine Triphosphatases
Organism Cloning
Complementary DNA

Keywords

  • Carbonic anhydrase
  • Cystine transport
  • Cystinuria
  • Ecto-adenosinetriphosphatase
  • GLUT2
  • Immunocytochemistry
  • In situ hybridization
  • Kidney

ASJC Scopus subject areas

  • Physiology

Cite this

Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule. / Kanai, Yoshikatsu; Stelzner, Matthias G.; Lee, Wen Sen; Wells, Rebecca G.; Brown, Dennis; Hediger, Matthias A.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 263, No. 6 32-6, 12.1992.

Research output: Contribution to journalArticle

Kanai, Yoshikatsu ; Stelzner, Matthias G. ; Lee, Wen Sen ; Wells, Rebecca G. ; Brown, Dennis ; Hediger, Matthias A. / Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1992 ; Vol. 263, No. 6 32-6.
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