Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages

Ming-Shium Hsieh, Hsin Chiu Ho, Der Tsay Chou, Shiann Pan, Yu-Chih Liang, Tsu Yi Hsieh, Joung Liang Lan, Shu-Huei Tsai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To investigate the relevance of gelatinase-B (matrix metalloproteinase 9, MMP-9) in gouty arthritis (GA), we tested the occurrence of MMP-9 in GA patients and cell culture system. Gelatinolytic activity in the synovial fluid (SF) of patients with different kinds of arthritis was assessed by gelatin zymography. A predominant 92-kDa MMP-9 gelatinolytic activity was evident in rheumatoid arthritis (RA) and GA samples, but no activity was observed in osteoarthritis (OA) samples. Among the 53 SF samples (9 RA, 24 GA, and 20 OA) analyzed for MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1) antigen levels by ELISA, MMP-9 antigen levels were elevated tenfold in GA SF compared with OA SF. In addition, GA synovial tissue extracts revealed elevated levels of MMP-9 expression as compared to OA tissue extracts by Western blot and RT-PCR analysis. Immunohistochemical studies demonstrated that MMP-9 immunoreactivity was more intense in GA than in OA synovial tissues. Furthermore, macrophages activation by gouty crystals in vitro was examined. Crystals stimulated MMP-9 gene expression in macrophage cell line and such stimulation was suppressed by PD98059. These findings suggest that the abnormal production of MMP-9 by macrophages is a reflection of the pathological conditions in joints of patients with GA, and that the activation of MMP-9 in the joint is known to play an important role in joint disease.

Original languageEnglish
Pages (from-to)791-799
Number of pages9
JournalJournal of Cellular Biochemistry
Volume89
Issue number4
DOIs
Publication statusPublished - Jul 1 2003

Fingerprint

Gouty Arthritis
Macrophages
Matrix Metalloproteinase 9
Uric Acid
Crystals
Osteoarthritis
Synovial Fluid
Tissue Inhibitor of Metalloproteinase-1
Tissue Extracts
Fluids
Rheumatoid Arthritis
Joints
Chemical activation
Tissue Inhibitor of Metalloproteinases
Antigens
Matrix Metalloproteinase Inhibitors
Macrophage Activation
Joint Diseases
Gelatin
Cell culture

Keywords

  • Gouty arthritis
  • MMP-9
  • MSU

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages. / Hsieh, Ming-Shium; Ho, Hsin Chiu; Chou, Der Tsay; Pan, Shiann; Liang, Yu-Chih; Hsieh, Tsu Yi; Lan, Joung Liang; Tsai, Shu-Huei.

In: Journal of Cellular Biochemistry, Vol. 89, No. 4, 01.07.2003, p. 791-799.

Research output: Contribution to journalArticle

Hsieh, Ming-Shium ; Ho, Hsin Chiu ; Chou, Der Tsay ; Pan, Shiann ; Liang, Yu-Chih ; Hsieh, Tsu Yi ; Lan, Joung Liang ; Tsai, Shu-Huei. / Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages. In: Journal of Cellular Biochemistry. 2003 ; Vol. 89, No. 4. pp. 791-799.
@article{e4e03fa379a44f84a7c9aa0aeded403d,
title = "Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages",
abstract = "To investigate the relevance of gelatinase-B (matrix metalloproteinase 9, MMP-9) in gouty arthritis (GA), we tested the occurrence of MMP-9 in GA patients and cell culture system. Gelatinolytic activity in the synovial fluid (SF) of patients with different kinds of arthritis was assessed by gelatin zymography. A predominant 92-kDa MMP-9 gelatinolytic activity was evident in rheumatoid arthritis (RA) and GA samples, but no activity was observed in osteoarthritis (OA) samples. Among the 53 SF samples (9 RA, 24 GA, and 20 OA) analyzed for MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1) antigen levels by ELISA, MMP-9 antigen levels were elevated tenfold in GA SF compared with OA SF. In addition, GA synovial tissue extracts revealed elevated levels of MMP-9 expression as compared to OA tissue extracts by Western blot and RT-PCR analysis. Immunohistochemical studies demonstrated that MMP-9 immunoreactivity was more intense in GA than in OA synovial tissues. Furthermore, macrophages activation by gouty crystals in vitro was examined. Crystals stimulated MMP-9 gene expression in macrophage cell line and such stimulation was suppressed by PD98059. These findings suggest that the abnormal production of MMP-9 by macrophages is a reflection of the pathological conditions in joints of patients with GA, and that the activation of MMP-9 in the joint is known to play an important role in joint disease.",
keywords = "Gouty arthritis, MMP-9, MSU",
author = "Ming-Shium Hsieh and Ho, {Hsin Chiu} and Chou, {Der Tsay} and Shiann Pan and Yu-Chih Liang and Hsieh, {Tsu Yi} and Lan, {Joung Liang} and Shu-Huei Tsai",
year = "2003",
month = "7",
day = "1",
doi = "10.1002/jcb.10530",
language = "English",
volume = "89",
pages = "791--799",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Expression of matrix metalloproteinase-9 (gelatinase B) in gouty arthritis and stimulation of MMP-9 by urate crystals in macrophages

AU - Hsieh, Ming-Shium

AU - Ho, Hsin Chiu

AU - Chou, Der Tsay

AU - Pan, Shiann

AU - Liang, Yu-Chih

AU - Hsieh, Tsu Yi

AU - Lan, Joung Liang

AU - Tsai, Shu-Huei

PY - 2003/7/1

Y1 - 2003/7/1

N2 - To investigate the relevance of gelatinase-B (matrix metalloproteinase 9, MMP-9) in gouty arthritis (GA), we tested the occurrence of MMP-9 in GA patients and cell culture system. Gelatinolytic activity in the synovial fluid (SF) of patients with different kinds of arthritis was assessed by gelatin zymography. A predominant 92-kDa MMP-9 gelatinolytic activity was evident in rheumatoid arthritis (RA) and GA samples, but no activity was observed in osteoarthritis (OA) samples. Among the 53 SF samples (9 RA, 24 GA, and 20 OA) analyzed for MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1) antigen levels by ELISA, MMP-9 antigen levels were elevated tenfold in GA SF compared with OA SF. In addition, GA synovial tissue extracts revealed elevated levels of MMP-9 expression as compared to OA tissue extracts by Western blot and RT-PCR analysis. Immunohistochemical studies demonstrated that MMP-9 immunoreactivity was more intense in GA than in OA synovial tissues. Furthermore, macrophages activation by gouty crystals in vitro was examined. Crystals stimulated MMP-9 gene expression in macrophage cell line and such stimulation was suppressed by PD98059. These findings suggest that the abnormal production of MMP-9 by macrophages is a reflection of the pathological conditions in joints of patients with GA, and that the activation of MMP-9 in the joint is known to play an important role in joint disease.

AB - To investigate the relevance of gelatinase-B (matrix metalloproteinase 9, MMP-9) in gouty arthritis (GA), we tested the occurrence of MMP-9 in GA patients and cell culture system. Gelatinolytic activity in the synovial fluid (SF) of patients with different kinds of arthritis was assessed by gelatin zymography. A predominant 92-kDa MMP-9 gelatinolytic activity was evident in rheumatoid arthritis (RA) and GA samples, but no activity was observed in osteoarthritis (OA) samples. Among the 53 SF samples (9 RA, 24 GA, and 20 OA) analyzed for MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1) antigen levels by ELISA, MMP-9 antigen levels were elevated tenfold in GA SF compared with OA SF. In addition, GA synovial tissue extracts revealed elevated levels of MMP-9 expression as compared to OA tissue extracts by Western blot and RT-PCR analysis. Immunohistochemical studies demonstrated that MMP-9 immunoreactivity was more intense in GA than in OA synovial tissues. Furthermore, macrophages activation by gouty crystals in vitro was examined. Crystals stimulated MMP-9 gene expression in macrophage cell line and such stimulation was suppressed by PD98059. These findings suggest that the abnormal production of MMP-9 by macrophages is a reflection of the pathological conditions in joints of patients with GA, and that the activation of MMP-9 in the joint is known to play an important role in joint disease.

KW - Gouty arthritis

KW - MMP-9

KW - MSU

UR - http://www.scopus.com/inward/record.url?scp=0037523502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037523502&partnerID=8YFLogxK

U2 - 10.1002/jcb.10530

DO - 10.1002/jcb.10530

M3 - Article

C2 - 12858344

AN - SCOPUS:0037523502

VL - 89

SP - 791

EP - 799

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 4

ER -