Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy

Hsin Fu Chen, Kuang Han Chao, Jin Yuh Shew, Yu Shih Yang, Hong Nerng Ho

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Uterine expression of leukemia inhibitory factor (LIF) is absolutely essential for mouse, and critical for human, embryo implantation. However LIF is not required for post-implantation development of mouse embryo. The objective of this study was to examine the role of LIF system in post-implantation stage of human pregnancy. Methods: Tissues from 25 patients with anembryonic pregnancy (AP; blighted ovum) and 25 matched patients with normal pregnancy (NP) were collected. LIF and its receptor β (LIF-Rβ) expression in the decidua and chorionic villi were analyzed by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR), real-time quantitative PCR and immunohistochemical study. Results: LIF mRNA levels were not different either between different tissues (decidua vs chorionic villi) or between different patients (NP vs AP). LIF-Rβ mRNA levels were significantly higher in chorionic villi than in decidua but were not different between NP and AP. Immunohistochemical staining supported these findings and showed a predominate expression of LIF-Rβ in the trophoblast cells. Conclusions: This study concluded that at early human post-implantation stage, LIF is produced from both decidua and chorionic villi and may exert its major action on trophoblasts. A baseline expression of LIF and LIF-Rβ is probably needed for early pregnancy, but AP cannot be accounted for by the defective expression of either LIF or LIF-Rβ in most circumstances.

Original languageEnglish
Pages (from-to)1647-1654
Number of pages8
JournalHuman Reproduction
Volume19
Issue number7
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

Fingerprint

Leukemia Inhibitory Factor Receptor alpha Subunit
OSM-LIF Receptors
Leukemia Inhibitory Factor
Chorionic Villi
Decidua
Cytokine Receptors
Pregnancy Complications
Computer Systems
Case-Control Studies
Interleukin-6
Immunohistochemistry
Gene Expression
Pregnancy
Polymerase Chain Reaction
Messenger RNA
Trophoblasts

Keywords

  • Anembryonic pregnancy
  • Blighted ovum
  • Chorionic villi
  • Decidua
  • Leukemia inhibitory factor

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy. / Chen, Hsin Fu; Chao, Kuang Han; Shew, Jin Yuh; Yang, Yu Shih; Ho, Hong Nerng.

In: Human Reproduction, Vol. 19, No. 7, 01.01.2004, p. 1647-1654.

Research output: Contribution to journalArticle

@article{cd6bb0c7e79f42848237e4e0c0e17c25,
title = "Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy",
abstract = "Background: Uterine expression of leukemia inhibitory factor (LIF) is absolutely essential for mouse, and critical for human, embryo implantation. However LIF is not required for post-implantation development of mouse embryo. The objective of this study was to examine the role of LIF system in post-implantation stage of human pregnancy. Methods: Tissues from 25 patients with anembryonic pregnancy (AP; blighted ovum) and 25 matched patients with normal pregnancy (NP) were collected. LIF and its receptor β (LIF-Rβ) expression in the decidua and chorionic villi were analyzed by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR), real-time quantitative PCR and immunohistochemical study. Results: LIF mRNA levels were not different either between different tissues (decidua vs chorionic villi) or between different patients (NP vs AP). LIF-Rβ mRNA levels were significantly higher in chorionic villi than in decidua but were not different between NP and AP. Immunohistochemical staining supported these findings and showed a predominate expression of LIF-Rβ in the trophoblast cells. Conclusions: This study concluded that at early human post-implantation stage, LIF is produced from both decidua and chorionic villi and may exert its major action on trophoblasts. A baseline expression of LIF and LIF-Rβ is probably needed for early pregnancy, but AP cannot be accounted for by the defective expression of either LIF or LIF-Rβ in most circumstances.",
keywords = "Anembryonic pregnancy, Blighted ovum, Chorionic villi, Decidua, Leukemia inhibitory factor",
author = "Chen, {Hsin Fu} and Chao, {Kuang Han} and Shew, {Jin Yuh} and Yang, {Yu Shih} and Ho, {Hong Nerng}",
year = "2004",
month = "1",
day = "1",
doi = "10.1093/humrep/deh193",
language = "English",
volume = "19",
pages = "1647--1654",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Expression of leukemia inhibitory factor and its receptor is not altered in the decidua and chorionic villi of human anembryonic pregnancy

AU - Chen, Hsin Fu

AU - Chao, Kuang Han

AU - Shew, Jin Yuh

AU - Yang, Yu Shih

AU - Ho, Hong Nerng

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Background: Uterine expression of leukemia inhibitory factor (LIF) is absolutely essential for mouse, and critical for human, embryo implantation. However LIF is not required for post-implantation development of mouse embryo. The objective of this study was to examine the role of LIF system in post-implantation stage of human pregnancy. Methods: Tissues from 25 patients with anembryonic pregnancy (AP; blighted ovum) and 25 matched patients with normal pregnancy (NP) were collected. LIF and its receptor β (LIF-Rβ) expression in the decidua and chorionic villi were analyzed by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR), real-time quantitative PCR and immunohistochemical study. Results: LIF mRNA levels were not different either between different tissues (decidua vs chorionic villi) or between different patients (NP vs AP). LIF-Rβ mRNA levels were significantly higher in chorionic villi than in decidua but were not different between NP and AP. Immunohistochemical staining supported these findings and showed a predominate expression of LIF-Rβ in the trophoblast cells. Conclusions: This study concluded that at early human post-implantation stage, LIF is produced from both decidua and chorionic villi and may exert its major action on trophoblasts. A baseline expression of LIF and LIF-Rβ is probably needed for early pregnancy, but AP cannot be accounted for by the defective expression of either LIF or LIF-Rβ in most circumstances.

AB - Background: Uterine expression of leukemia inhibitory factor (LIF) is absolutely essential for mouse, and critical for human, embryo implantation. However LIF is not required for post-implantation development of mouse embryo. The objective of this study was to examine the role of LIF system in post-implantation stage of human pregnancy. Methods: Tissues from 25 patients with anembryonic pregnancy (AP; blighted ovum) and 25 matched patients with normal pregnancy (NP) were collected. LIF and its receptor β (LIF-Rβ) expression in the decidua and chorionic villi were analyzed by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR), real-time quantitative PCR and immunohistochemical study. Results: LIF mRNA levels were not different either between different tissues (decidua vs chorionic villi) or between different patients (NP vs AP). LIF-Rβ mRNA levels were significantly higher in chorionic villi than in decidua but were not different between NP and AP. Immunohistochemical staining supported these findings and showed a predominate expression of LIF-Rβ in the trophoblast cells. Conclusions: This study concluded that at early human post-implantation stage, LIF is produced from both decidua and chorionic villi and may exert its major action on trophoblasts. A baseline expression of LIF and LIF-Rβ is probably needed for early pregnancy, but AP cannot be accounted for by the defective expression of either LIF or LIF-Rβ in most circumstances.

KW - Anembryonic pregnancy

KW - Blighted ovum

KW - Chorionic villi

KW - Decidua

KW - Leukemia inhibitory factor

UR - http://www.scopus.com/inward/record.url?scp=3242875926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242875926&partnerID=8YFLogxK

U2 - 10.1093/humrep/deh193

DO - 10.1093/humrep/deh193

M3 - Article

C2 - 15180980

AN - SCOPUS:3242875926

VL - 19

SP - 1647

EP - 1654

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 7

ER -