Expression of leukemia inhibitory factor and its receptor in preimplantation embryos

Hsin Fu Chen, Jin Yuh Shew, Hong Nerng Ho, Wei Li Hsu, Yu Shih Yang

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Objective: To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos. Design: Prospective study. Setting: University medical center. Patient(s): Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice. Intervention(s): Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study. Main Outcome Measure(s): The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology. Result(s): Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent. Conclusion(s): Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.

Original languageEnglish
Pages (from-to)713-719
Number of pages7
JournalFertility and Sterility
Volume72
Issue number4
DOIs
Publication statusPublished - Oct 1 1999
Externally publishedYes

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Leukemia Inhibitory Factor Receptor alpha Subunit
OSM-LIF Receptors
Leukemia Inhibitory Factor
Growth Inhibitors
Inbred ICR Mouse
Cytokine Receptors
Lymphokines
Blastocyst
Southern Blotting
Interleukin-6
Prospective Studies
Cytokines
Polymerase Chain Reaction
Messenger RNA
Embryonic Structures
Oocytes
vpr Genes
Intracytoplasmic Sperm Injections

Keywords

  • Cytokine
  • Implantation
  • Leukemia inhibitory factor
  • Leukemia inhibitory factor receptor
  • Preimplantation embryo

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Expression of leukemia inhibitory factor and its receptor in preimplantation embryos. / Chen, Hsin Fu; Shew, Jin Yuh; Ho, Hong Nerng; Hsu, Wei Li; Yang, Yu Shih.

In: Fertility and Sterility, Vol. 72, No. 4, 01.10.1999, p. 713-719.

Research output: Contribution to journalArticle

Chen, Hsin Fu ; Shew, Jin Yuh ; Ho, Hong Nerng ; Hsu, Wei Li ; Yang, Yu Shih. / Expression of leukemia inhibitory factor and its receptor in preimplantation embryos. In: Fertility and Sterility. 1999 ; Vol. 72, No. 4. pp. 713-719.
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