Expression of Epstein-Barr virus in carcinomas of major salivary glands: A strong association with lymphoepithelioma-like carcinoma

Chien Chen Tsai, Chi Long Chen, Hey C. Hsu

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

To elucidate the role of Epstein-Barr virus (EBV) in salivary gland carcinomas, 56 cases of carcinomas of major salivary glands were investigated. These included 14 mucoepidermoid carcinomas, 13 adenoid cystic carcinomas, seven malignant mixed tumors, four adenocarcinomas, four salivary duct carcinomas, two acinic cell carcinomas, two undifferentiated carcinomas without lymphoid stroma, seven lymphoepithelioma-like carcinomas (LELCs), two squamous cell carcinomas, and one small cell carcinoma. EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin- embedded tissue sections. EBER1 was detected in the malignant epithelial cells in all seven cases of LELC, but not in any of the other carcinomas and the neighboring normal salivary gland tissue. Because all the EBV-negative cases showed satisfactory labeling with the poly d(T) probe, the negative reaction with EBER1 was unlikely to be caused by poor RNA preservation in the tissues. The seven cases of LELC, which were histologically indistinguishable from undifferentiated nasopharyngeal carcinoma (NPC), had a disease-free 4- year survival rate of 85.7%. The results suggest that LELC of the salivary gland in Taiwanese Chinese may share similar EBV-related pathogenesis with that of NPC.

Original languageEnglish
Pages (from-to)258-262
Number of pages5
JournalHuman Pathology
Volume27
Issue number3
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Salivary Glands
Human Herpesvirus 4
Carcinoma
RNA
Malignant Mixed Tumor
Poly T
Acinar Cell Carcinoma
Salivary Ducts
Tissue Preservation
Mucoepidermoid Carcinoma
Digoxigenin
Adenoid Cystic Carcinoma
Small Cell Carcinoma
Oligonucleotide Probes
Paraffin
Formaldehyde
In Situ Hybridization
Squamous Cell Carcinoma
Adenocarcinoma
Epithelial Cells

Keywords

  • Epstein-Barr virus
  • in situ hybridization
  • lymphoepithelioma-like carcinoma
  • salivary gland tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of Epstein-Barr virus in carcinomas of major salivary glands : A strong association with lymphoepithelioma-like carcinoma. / Tsai, Chien Chen; Chen, Chi Long; Hsu, Hey C.

In: Human Pathology, Vol. 27, No. 3, 1996, p. 258-262.

Research output: Contribution to journalArticle

@article{d8f4ec8187be491f8ca0a4c3562df6eb,
title = "Expression of Epstein-Barr virus in carcinomas of major salivary glands: A strong association with lymphoepithelioma-like carcinoma",
abstract = "To elucidate the role of Epstein-Barr virus (EBV) in salivary gland carcinomas, 56 cases of carcinomas of major salivary glands were investigated. These included 14 mucoepidermoid carcinomas, 13 adenoid cystic carcinomas, seven malignant mixed tumors, four adenocarcinomas, four salivary duct carcinomas, two acinic cell carcinomas, two undifferentiated carcinomas without lymphoid stroma, seven lymphoepithelioma-like carcinomas (LELCs), two squamous cell carcinomas, and one small cell carcinoma. EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin- embedded tissue sections. EBER1 was detected in the malignant epithelial cells in all seven cases of LELC, but not in any of the other carcinomas and the neighboring normal salivary gland tissue. Because all the EBV-negative cases showed satisfactory labeling with the poly d(T) probe, the negative reaction with EBER1 was unlikely to be caused by poor RNA preservation in the tissues. The seven cases of LELC, which were histologically indistinguishable from undifferentiated nasopharyngeal carcinoma (NPC), had a disease-free 4- year survival rate of 85.7{\%}. The results suggest that LELC of the salivary gland in Taiwanese Chinese may share similar EBV-related pathogenesis with that of NPC.",
keywords = "Epstein-Barr virus, in situ hybridization, lymphoepithelioma-like carcinoma, salivary gland tumor",
author = "Tsai, {Chien Chen} and Chen, {Chi Long} and Hsu, {Hey C.}",
year = "1996",
doi = "10.1016/S0046-8177(96)90066-0",
language = "English",
volume = "27",
pages = "258--262",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders",
number = "3",

}

TY - JOUR

T1 - Expression of Epstein-Barr virus in carcinomas of major salivary glands

T2 - A strong association with lymphoepithelioma-like carcinoma

AU - Tsai, Chien Chen

AU - Chen, Chi Long

AU - Hsu, Hey C.

PY - 1996

Y1 - 1996

N2 - To elucidate the role of Epstein-Barr virus (EBV) in salivary gland carcinomas, 56 cases of carcinomas of major salivary glands were investigated. These included 14 mucoepidermoid carcinomas, 13 adenoid cystic carcinomas, seven malignant mixed tumors, four adenocarcinomas, four salivary duct carcinomas, two acinic cell carcinomas, two undifferentiated carcinomas without lymphoid stroma, seven lymphoepithelioma-like carcinomas (LELCs), two squamous cell carcinomas, and one small cell carcinoma. EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin- embedded tissue sections. EBER1 was detected in the malignant epithelial cells in all seven cases of LELC, but not in any of the other carcinomas and the neighboring normal salivary gland tissue. Because all the EBV-negative cases showed satisfactory labeling with the poly d(T) probe, the negative reaction with EBER1 was unlikely to be caused by poor RNA preservation in the tissues. The seven cases of LELC, which were histologically indistinguishable from undifferentiated nasopharyngeal carcinoma (NPC), had a disease-free 4- year survival rate of 85.7%. The results suggest that LELC of the salivary gland in Taiwanese Chinese may share similar EBV-related pathogenesis with that of NPC.

AB - To elucidate the role of Epstein-Barr virus (EBV) in salivary gland carcinomas, 56 cases of carcinomas of major salivary glands were investigated. These included 14 mucoepidermoid carcinomas, 13 adenoid cystic carcinomas, seven malignant mixed tumors, four adenocarcinomas, four salivary duct carcinomas, two acinic cell carcinomas, two undifferentiated carcinomas without lymphoid stroma, seven lymphoepithelioma-like carcinomas (LELCs), two squamous cell carcinomas, and one small cell carcinoma. EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin- embedded tissue sections. EBER1 was detected in the malignant epithelial cells in all seven cases of LELC, but not in any of the other carcinomas and the neighboring normal salivary gland tissue. Because all the EBV-negative cases showed satisfactory labeling with the poly d(T) probe, the negative reaction with EBER1 was unlikely to be caused by poor RNA preservation in the tissues. The seven cases of LELC, which were histologically indistinguishable from undifferentiated nasopharyngeal carcinoma (NPC), had a disease-free 4- year survival rate of 85.7%. The results suggest that LELC of the salivary gland in Taiwanese Chinese may share similar EBV-related pathogenesis with that of NPC.

KW - Epstein-Barr virus

KW - in situ hybridization

KW - lymphoepithelioma-like carcinoma

KW - salivary gland tumor

UR - http://www.scopus.com/inward/record.url?scp=0029877082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029877082&partnerID=8YFLogxK

U2 - 10.1016/S0046-8177(96)90066-0

DO - 10.1016/S0046-8177(96)90066-0

M3 - Article

C2 - 8600040

AN - SCOPUS:0029877082

VL - 27

SP - 258

EP - 262

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 3

ER -