Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection

Shin Ru Shih, Yi Shuane Li, Chiuan Chian Chiou, Pin Chau Suen, Tzou Yien Lin, Luan Yin Chang, Yhu Chering Huang, Kuo Chien Tsao, Hsiao Chen Ning, Tzong Zeng Wu, Err Cheng Chan

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti-VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection. In addition, sera containing coxsackie virus 16 (CA16) antibodies did not cross-react with the recombinant VP1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalJournal of Medical Virology
Volume61
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Enterovirus Infections
Enterovirus
Antigens
Immunoglobulin G
Serum
Immunoglobulin M
Antibodies
Proteins
Polymerase Chain Reaction
Capsid Proteins
Serologic Tests
Infection
Reverse Transcription
Epitopes
Western Blotting
Escherichia coli
Viruses
Peptides
Genes

Keywords

  • Acute infections
  • Enterovirus 71
  • Enterovirus 71 antibody
  • Past infections
  • Recombinant capsid protein VP1
  • Serodiagnosis

ASJC Scopus subject areas

  • Virology

Cite this

Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection. / Shih, Shin Ru; Li, Yi Shuane; Chiou, Chiuan Chian; Suen, Pin Chau; Lin, Tzou Yien; Chang, Luan Yin; Huang, Yhu Chering; Tsao, Kuo Chien; Ning, Hsiao Chen; Wu, Tzong Zeng; Chan, Err Cheng.

In: Journal of Medical Virology, Vol. 61, No. 2, 2000, p. 228-234.

Research output: Contribution to journalArticle

Shih, SR, Li, YS, Chiou, CC, Suen, PC, Lin, TY, Chang, LY, Huang, YC, Tsao, KC, Ning, HC, Wu, TZ & Chan, EC 2000, 'Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection', Journal of Medical Virology, vol. 61, no. 2, pp. 228-234. https://doi.org/10.1002/(SICI)1096-9071(200006)61:2<228::AID-JMV9>3.0.CO;2-R
Shih, Shin Ru ; Li, Yi Shuane ; Chiou, Chiuan Chian ; Suen, Pin Chau ; Lin, Tzou Yien ; Chang, Luan Yin ; Huang, Yhu Chering ; Tsao, Kuo Chien ; Ning, Hsiao Chen ; Wu, Tzong Zeng ; Chan, Err Cheng. / Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection. In: Journal of Medical Virology. 2000 ; Vol. 61, No. 2. pp. 228-234.
@article{913fdcb685d94470bcb3048699f64870,
title = "Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection",
abstract = "To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti-VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection. In addition, sera containing coxsackie virus 16 (CA16) antibodies did not cross-react with the recombinant VP1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection. (C) 2000 Wiley-Liss, Inc.",
keywords = "Acute infections, Enterovirus 71, Enterovirus 71 antibody, Past infections, Recombinant capsid protein VP1, Serodiagnosis",
author = "Shih, {Shin Ru} and Li, {Yi Shuane} and Chiou, {Chiuan Chian} and Suen, {Pin Chau} and Lin, {Tzou Yien} and Chang, {Luan Yin} and Huang, {Yhu Chering} and Tsao, {Kuo Chien} and Ning, {Hsiao Chen} and Wu, {Tzong Zeng} and Chan, {Err Cheng}",
year = "2000",
doi = "10.1002/(SICI)1096-9071(200006)61:2<228::AID-JMV9>3.0.CO;2-R",
language = "English",
volume = "61",
pages = "228--234",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection

AU - Shih, Shin Ru

AU - Li, Yi Shuane

AU - Chiou, Chiuan Chian

AU - Suen, Pin Chau

AU - Lin, Tzou Yien

AU - Chang, Luan Yin

AU - Huang, Yhu Chering

AU - Tsao, Kuo Chien

AU - Ning, Hsiao Chen

AU - Wu, Tzong Zeng

AU - Chan, Err Cheng

PY - 2000

Y1 - 2000

N2 - To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti-VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection. In addition, sera containing coxsackie virus 16 (CA16) antibodies did not cross-react with the recombinant VP1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection. (C) 2000 Wiley-Liss, Inc.

AB - To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti-VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection. In addition, sera containing coxsackie virus 16 (CA16) antibodies did not cross-react with the recombinant VP1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection. (C) 2000 Wiley-Liss, Inc.

KW - Acute infections

KW - Enterovirus 71

KW - Enterovirus 71 antibody

KW - Past infections

KW - Recombinant capsid protein VP1

KW - Serodiagnosis

UR - http://www.scopus.com/inward/record.url?scp=0034203444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034203444&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9071(200006)61:2<228::AID-JMV9>3.0.CO;2-R

DO - 10.1002/(SICI)1096-9071(200006)61:2<228::AID-JMV9>3.0.CO;2-R

M3 - Article

C2 - 10797379

AN - SCOPUS:0034203444

VL - 61

SP - 228

EP - 234

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -