Expression of aminopeptidase N in bile canaliculi

A predictor of clinical outcome in biliary atresia and a potential tool to implicate the mechanism of biliary atresia

Chinsu Liu, Jen Hwey Chiu, Taiwai Chin, Liang Shun Wang, Chih Ho Tai, Anna Fen Yau Li, Choufu Wei

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background. Only a few studies on extrahepatic biliary atresia (BA) have reported that the morphological changes of bile canaliculi could predict the clinical outcome after portoenterostomy and provide differential diagnosis of neonatal jaundice. Aminopeptidase N (APN) is an ectoenzyme of bile canaliculi that is involved in bile secretion. In this study, we tried to see whether APN of bile canaliculi had a significant role in BA. Patients and methods. We used monoclonal antibody 9B2 to compare the expression of APN in livers with BA, neonatal hepatitis, and choledochal cysts, as well as in nontumorous portions of pediatric hepatic livers with tumors. The expression of APN in fetuses, preterm babies, and term neonates was also studied. Results. A high degree of 9B2 expression in BA was closely related to poor outcome. Cholestasis in choledochal cysts, rather than neonatal hepatitis, made 9B2 expression stronger. Increasing expression of 9B2 from fetuses to neonates was noted and the degree of 9B2 expression was similar between term neonates and nontumorous portions of pediatric livers with tumors. Interestingly, some cases of BA had 9B2 expression like that of preterm babies. Conclusions. APN of bile canaliculi progressively develops from fetuses to neonates and is well developed in neonates. APN can be induced to stronger expression by obstructive jaundice. The amount of expression of APN of bile canaliculi in BA is a predictor of clinical outcome and may be a tool for implicating the mechanism of BA.

Original languageEnglish
Pages (from-to)76-83
Number of pages8
JournalJournal of Surgical Research
Volume100
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Bile Canaliculi
CD13 Antigens
Biliary Atresia
Newborn Infant
Choledochal Cyst
Fetus
Liver
Hepatitis
Pediatrics
Neonatal Jaundice
Obstructive Jaundice
Cholestasis
Bile
Neoplasms
Differential Diagnosis
Monoclonal Antibodies

Keywords

  • Aminopeptidase N (APN)
  • Bile canaliculi
  • Biliary atresia
  • Fetus

ASJC Scopus subject areas

  • Surgery

Cite this

Expression of aminopeptidase N in bile canaliculi : A predictor of clinical outcome in biliary atresia and a potential tool to implicate the mechanism of biliary atresia. / Liu, Chinsu; Chiu, Jen Hwey; Chin, Taiwai; Wang, Liang Shun; Tai, Chih Ho; Li, Anna Fen Yau; Wei, Choufu.

In: Journal of Surgical Research, Vol. 100, No. 1, 2001, p. 76-83.

Research output: Contribution to journalArticle

Liu, Chinsu ; Chiu, Jen Hwey ; Chin, Taiwai ; Wang, Liang Shun ; Tai, Chih Ho ; Li, Anna Fen Yau ; Wei, Choufu. / Expression of aminopeptidase N in bile canaliculi : A predictor of clinical outcome in biliary atresia and a potential tool to implicate the mechanism of biliary atresia. In: Journal of Surgical Research. 2001 ; Vol. 100, No. 1. pp. 76-83.
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abstract = "Background. Only a few studies on extrahepatic biliary atresia (BA) have reported that the morphological changes of bile canaliculi could predict the clinical outcome after portoenterostomy and provide differential diagnosis of neonatal jaundice. Aminopeptidase N (APN) is an ectoenzyme of bile canaliculi that is involved in bile secretion. In this study, we tried to see whether APN of bile canaliculi had a significant role in BA. Patients and methods. We used monoclonal antibody 9B2 to compare the expression of APN in livers with BA, neonatal hepatitis, and choledochal cysts, as well as in nontumorous portions of pediatric hepatic livers with tumors. The expression of APN in fetuses, preterm babies, and term neonates was also studied. Results. A high degree of 9B2 expression in BA was closely related to poor outcome. Cholestasis in choledochal cysts, rather than neonatal hepatitis, made 9B2 expression stronger. Increasing expression of 9B2 from fetuses to neonates was noted and the degree of 9B2 expression was similar between term neonates and nontumorous portions of pediatric livers with tumors. Interestingly, some cases of BA had 9B2 expression like that of preterm babies. Conclusions. APN of bile canaliculi progressively develops from fetuses to neonates and is well developed in neonates. APN can be induced to stronger expression by obstructive jaundice. The amount of expression of APN of bile canaliculi in BA is a predictor of clinical outcome and may be a tool for implicating the mechanism of BA.",
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AU - Chin, Taiwai

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AU - Tai, Chih Ho

AU - Li, Anna Fen Yau

AU - Wei, Choufu

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N2 - Background. Only a few studies on extrahepatic biliary atresia (BA) have reported that the morphological changes of bile canaliculi could predict the clinical outcome after portoenterostomy and provide differential diagnosis of neonatal jaundice. Aminopeptidase N (APN) is an ectoenzyme of bile canaliculi that is involved in bile secretion. In this study, we tried to see whether APN of bile canaliculi had a significant role in BA. Patients and methods. We used monoclonal antibody 9B2 to compare the expression of APN in livers with BA, neonatal hepatitis, and choledochal cysts, as well as in nontumorous portions of pediatric hepatic livers with tumors. The expression of APN in fetuses, preterm babies, and term neonates was also studied. Results. A high degree of 9B2 expression in BA was closely related to poor outcome. Cholestasis in choledochal cysts, rather than neonatal hepatitis, made 9B2 expression stronger. Increasing expression of 9B2 from fetuses to neonates was noted and the degree of 9B2 expression was similar between term neonates and nontumorous portions of pediatric livers with tumors. Interestingly, some cases of BA had 9B2 expression like that of preterm babies. Conclusions. APN of bile canaliculi progressively develops from fetuses to neonates and is well developed in neonates. APN can be induced to stronger expression by obstructive jaundice. The amount of expression of APN of bile canaliculi in BA is a predictor of clinical outcome and may be a tool for implicating the mechanism of BA.

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