Expression of a novel TGIF subclass homeobox gene, Tex1, in the spermatids of mouse testis during spermatogenesis

Yi Lun Lai, Hung Li, Han-Sun Chiang, Hsiu Mei Hsieh-Li

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The homeobox gene superfamily is highly conserved during evolution. These genes act as transcription factors during several important developmental processes through their 60 amino acid homeodomains that recognize and bind to the regulatory region of their target genes. We isolated a novel homeobox gene designated Tex1 from a mouse testis cDNA library by degenerate oligonucleotide polymerase chain reaction (PCR) screening. Three extra amino acid residues, AYP, are inserted between the first and second helices of the homeodomain, which makes Tex1 a TGIF subclass homeobox gene. Tex1 was expressed specifically in the testis as seen by both Northern blot and RT-PCR analysis. Tex1 expression is initiated at around day 20 of testes. Furthermore, the results of in situ hybridization analysis reveal that the expression of Tex1 in testis is restricted in the germ cells at spermatid stage. Based on these data, it is suggested that Tex1, a novel testis homeobox gene, may play a crucial role during spermatogenesis.

Original languageEnglish
Pages (from-to)185-187
Number of pages3
JournalMechanisms of Development
Volume113
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Spermatids
Homeobox Genes
Spermatogenesis
Testis
Amino Acids
Polymerase Chain Reaction
Nucleic Acid Regulatory Sequences
Gene Library
Germ Cells
Northern Blotting
Genes
In Situ Hybridization
Transcription Factors

Keywords

  • Homeobox
  • Mouse
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Expression of a novel TGIF subclass homeobox gene, Tex1, in the spermatids of mouse testis during spermatogenesis. / Lai, Yi Lun; Li, Hung; Chiang, Han-Sun; Hsieh-Li, Hsiu Mei.

In: Mechanisms of Development, Vol. 113, No. 2, 2002, p. 185-187.

Research output: Contribution to journalArticle

Lai, Yi Lun ; Li, Hung ; Chiang, Han-Sun ; Hsieh-Li, Hsiu Mei. / Expression of a novel TGIF subclass homeobox gene, Tex1, in the spermatids of mouse testis during spermatogenesis. In: Mechanisms of Development. 2002 ; Vol. 113, No. 2. pp. 185-187.
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