Expression levels of vascular cell adhesion molecule-1 in young and nonobese women with polycystic ovary syndrome

Kok Min Seow, Chi Chang Juan, Peng Hui Wang, Low Tone Ho, Jiann Loung Hwang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background/Aims: The objective of this study was to measure levels of mRNAs for inflammatory markers and resistin in human peripheral blood mononuclear cells (PBMCs) in young and nonobese women with polycystic ovary syndrome (PCOS). Methods: Fifteen young, nonobese women with PCOS and 10 age-matched controls were enrolled in this study. Levels of mRNAs for resistin and the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human PBMCs were measured using real-time PCR. Results: The mean age and BMI of the women with PCOS were 27.54 ± 6.3 years and 27.4 ± 5.7, respectively. The women with PCOS had significantly higher fasting and 2-hour insulin levels, homeostasis model assessment of insulin resistance index (HOMA IR) and total cholesterol levels than the controls. VCAM-1 and ICAM-1 mRNA levels were significantly higher in women with PCOS than in controls, whereas no differences in resistin, IL-6, TNF-α and MCP-1 mRNA levels were observed between the groups. After adjusting for the BMI, only VCAM-1 mRNA levels were significantly higher in women with PCOS than in controls and correlated with the HOMA IR and total cholesterol. Conclusion: Elevated VCAM-1 in human PBMCs in young, nonobese women with PCOS is associated with insulin resistance, independent of the BMI.

Original languageEnglish
Pages (from-to)236-241
Number of pages6
JournalGynecologic and Obstetric Investigation
Volume73
Issue number3
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Vascular Cell Adhesion Molecule-1
Polycystic Ovary Syndrome
Messenger RNA
Resistin
Insulin Resistance
Blood Cells
Chemokine CCL2
Intercellular Adhesion Molecule-1
Interleukin-6
Homeostasis
Tumor Necrosis Factor-alpha
Cholesterol
Real-Time Polymerase Chain Reaction
Fasting
Insulin

Keywords

  • Atherosclerosis
  • Inflammatory markers
  • Insulin resistance
  • Polycystic ovary syndrome
  • Resistin

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Expression levels of vascular cell adhesion molecule-1 in young and nonobese women with polycystic ovary syndrome. / Seow, Kok Min; Juan, Chi Chang; Wang, Peng Hui; Ho, Low Tone; Hwang, Jiann Loung.

In: Gynecologic and Obstetric Investigation, Vol. 73, No. 3, 04.2012, p. 236-241.

Research output: Contribution to journalArticle

Seow, Kok Min ; Juan, Chi Chang ; Wang, Peng Hui ; Ho, Low Tone ; Hwang, Jiann Loung. / Expression levels of vascular cell adhesion molecule-1 in young and nonobese women with polycystic ovary syndrome. In: Gynecologic and Obstetric Investigation. 2012 ; Vol. 73, No. 3. pp. 236-241.
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abstract = "Background/Aims: The objective of this study was to measure levels of mRNAs for inflammatory markers and resistin in human peripheral blood mononuclear cells (PBMCs) in young and nonobese women with polycystic ovary syndrome (PCOS). Methods: Fifteen young, nonobese women with PCOS and 10 age-matched controls were enrolled in this study. Levels of mRNAs for resistin and the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human PBMCs were measured using real-time PCR. Results: The mean age and BMI of the women with PCOS were 27.54 ± 6.3 years and 27.4 ± 5.7, respectively. The women with PCOS had significantly higher fasting and 2-hour insulin levels, homeostasis model assessment of insulin resistance index (HOMA IR) and total cholesterol levels than the controls. VCAM-1 and ICAM-1 mRNA levels were significantly higher in women with PCOS than in controls, whereas no differences in resistin, IL-6, TNF-α and MCP-1 mRNA levels were observed between the groups. After adjusting for the BMI, only VCAM-1 mRNA levels were significantly higher in women with PCOS than in controls and correlated with the HOMA IR and total cholesterol. Conclusion: Elevated VCAM-1 in human PBMCs in young, nonobese women with PCOS is associated with insulin resistance, independent of the BMI.",
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N2 - Background/Aims: The objective of this study was to measure levels of mRNAs for inflammatory markers and resistin in human peripheral blood mononuclear cells (PBMCs) in young and nonobese women with polycystic ovary syndrome (PCOS). Methods: Fifteen young, nonobese women with PCOS and 10 age-matched controls were enrolled in this study. Levels of mRNAs for resistin and the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human PBMCs were measured using real-time PCR. Results: The mean age and BMI of the women with PCOS were 27.54 ± 6.3 years and 27.4 ± 5.7, respectively. The women with PCOS had significantly higher fasting and 2-hour insulin levels, homeostasis model assessment of insulin resistance index (HOMA IR) and total cholesterol levels than the controls. VCAM-1 and ICAM-1 mRNA levels were significantly higher in women with PCOS than in controls, whereas no differences in resistin, IL-6, TNF-α and MCP-1 mRNA levels were observed between the groups. After adjusting for the BMI, only VCAM-1 mRNA levels were significantly higher in women with PCOS than in controls and correlated with the HOMA IR and total cholesterol. Conclusion: Elevated VCAM-1 in human PBMCs in young, nonobese women with PCOS is associated with insulin resistance, independent of the BMI.

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