Expression levels of haem oxygenase-1 in the omental adipose tissue and peripheral blood mononuclear cells of women with polycystic ovary syndrome

Kok Min Seow, Yu Hung Lin, Jiann Loung Hwang, Peng Hui Wang, Low Tone Ho, Yi Hsiu Lin, Chi Chang Juan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

BACKGROUNDHaem oxygenase (HO)-1, an enzyme that degrades haem, plays a key role in the regulation of the inflammatory response and insulin resistance. The aim of this study was to evaluate the role of HO-1 in the regulation of insulin resistance and glucose tolerance in women with polycystic ovary syndrome (PCOS).METHODSOmental adipose tissue and human peripheral blood mononuclear cells (PBMCs) from seven women with PCOS and five healthy controls, matched for BMI and age, were analysed using western blotting and the real-time PCR.RESULTS Women with PCOS were found to have significantly higher fasting and 2-h insulin levels, a significantly higher homeostasis model assessment insulin resistance index and a lower fasting glucose-to-insulin ratio (G0/I0) than the controls. The level of HO-1 protein in omental fat (P 0.002), and the expression of HO-1 mRNA in omental fat and PBMCs from the women with PCOS were significantly lower (P 0.002 and 0.05, respectively) than those of the controls. The expression of adiponectin mRNA in omental fat was also significantly lower (P 0.02) in the women with PCOS than in the controls. However, there were no significant differences in the expression of tumour necrosis factor-α or interleukin-6 between the two groups. The level of HO-1 protein showed a significant positive correlation with the expression of HO-1 mRNA (r2 0.786, P= 0.037) and adiponectin mRNA (r2 0.7276, P

Original languageEnglish
Pages (from-to)431-437
Number of pages7
JournalHuman Reproduction
Volume26
Issue number2
DOIs
Publication statusPublished - Feb 2011

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Heme Oxygenase (Decyclizing)
Polycystic Ovary Syndrome
Adipose Tissue
Blood Cells
Insulin Resistance
Messenger RNA
Fats
Adiponectin
Fasting
Insulin
Glucose
Oxygenases
Heme
Real-Time Polymerase Chain Reaction
Interleukin-6
Proteins
Homeostasis
Tumor Necrosis Factor-alpha
Western Blotting
Enzymes

Keywords

  • adiponectin
  • haem oxygenase-1
  • insulin resistance
  • polycystic ovary syndrome

ASJC Scopus subject areas

  • Rehabilitation
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Expression levels of haem oxygenase-1 in the omental adipose tissue and peripheral blood mononuclear cells of women with polycystic ovary syndrome. / Seow, Kok Min; Lin, Yu Hung; Hwang, Jiann Loung; Wang, Peng Hui; Ho, Low Tone; Lin, Yi Hsiu; Juan, Chi Chang.

In: Human Reproduction, Vol. 26, No. 2, 02.2011, p. 431-437.

Research output: Contribution to journalArticle

Seow, Kok Min ; Lin, Yu Hung ; Hwang, Jiann Loung ; Wang, Peng Hui ; Ho, Low Tone ; Lin, Yi Hsiu ; Juan, Chi Chang. / Expression levels of haem oxygenase-1 in the omental adipose tissue and peripheral blood mononuclear cells of women with polycystic ovary syndrome. In: Human Reproduction. 2011 ; Vol. 26, No. 2. pp. 431-437.
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