Expression and characterization of two STAT isoforms from Sf9 cells

Maw Sheng Yeh, Chia Hsiung Cheng, Chih Ming Chou, Ya Li Hsu, Cheng Ying Chu, Gen Der Chen, Shui Tsung Chen, Guang Chao Chen, Chang Jen Huang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.

Original languageEnglish
Pages (from-to)814-824
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume32
Issue number7
DOIs
Publication statusPublished - 2008

Fingerprint

Sf9 Cells
Protein Isoforms
Baculoviridae
Drosophila
Alternative Splicing
Invertebrates
Transcriptional Activation
Technology
DNA
Infection
Proteins

Keywords

  • Baculovirus
  • DNA binding
  • Insect Sf9 cells
  • STAT

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Expression and characterization of two STAT isoforms from Sf9 cells. / Yeh, Maw Sheng; Cheng, Chia Hsiung; Chou, Chih Ming; Hsu, Ya Li; Chu, Cheng Ying; Chen, Gen Der; Chen, Shui Tsung; Chen, Guang Chao; Huang, Chang Jen.

In: Developmental and Comparative Immunology, Vol. 32, No. 7, 2008, p. 814-824.

Research output: Contribution to journalArticle

Yeh, Maw Sheng ; Cheng, Chia Hsiung ; Chou, Chih Ming ; Hsu, Ya Li ; Chu, Cheng Ying ; Chen, Gen Der ; Chen, Shui Tsung ; Chen, Guang Chao ; Huang, Chang Jen. / Expression and characterization of two STAT isoforms from Sf9 cells. In: Developmental and Comparative Immunology. 2008 ; Vol. 32, No. 7. pp. 814-824.
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