Experimental focal segmental glomerulosclerosis in mice

Ann Chen, Lai Fa Sheu, Yat Sen Ho, Yu Fen Lin, Wei Yuan Chou, Tz Chong Chou, Wei Hwa Lee

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Although a lot of animal models of proteinuria have been established, proposals for the mechanisms of proteinuria are still controversial. In this work, during an 18-day trial, mice injected with a single dose of adriamycin (AD) rapidly showed combined glomerular albuminuria and immunoglobulinuria, progressively elevated levels of nitrite/nitrate in urine, hypercholesterolemia, abnormal renal function, segmentally or globally glomerular hyalinosis/sclerosis associated with tubular atrophy, enhanced glomerular deposition of immunoglobulins and fibrinogen, augmented expression of matrix components in the whole glomerular tuft, and loss of glomerular negative charge property. These laboratory and pathological features are comparatively similar to those of human focal segmental glomerulosclerosis in the advanced state. Juxtamedullary glomeruli appear to be more susceptible to the AD-related nephrotoxicity than those in the superficial renal cortex. A change in size-dependent glomerular permselectivity may precede a charge-dependent defect in glomeruli in this mouse model of proteinuria. Data in this study confirm the hypothesis of glomerular hyperfiltration involved in the pathogenesis of this chronic glomerulopathy associated with proteinuria in mice. In addition, nitric oxide may play a crucial role in the progression of the chronic glomerulopathy model.

Original languageEnglish
Pages (from-to)440-452
Number of pages13
JournalNephron
Volume78
Issue number4
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Focal Segmental Glomerulosclerosis
Proteinuria
Doxorubicin
Kidney
Albuminuria
Sclerosis
Nitrites
Hypercholesterolemia
Nitrates
Fibrinogen
Atrophy
Immunoglobulins
Nitric Oxide
Animal Models
Urine

Keywords

  • Adriamycin
  • Glomerulosclerosis
  • Mouse
  • Nitrate
  • Nitrite
  • Proteinuria

ASJC Scopus subject areas

  • Nephrology

Cite this

Chen, A., Sheu, L. F., Ho, Y. S., Lin, Y. F., Chou, W. Y., Chou, T. C., & Lee, W. H. (1998). Experimental focal segmental glomerulosclerosis in mice. Nephron, 78(4), 440-452. https://doi.org/10.1159/000044974

Experimental focal segmental glomerulosclerosis in mice. / Chen, Ann; Sheu, Lai Fa; Ho, Yat Sen; Lin, Yu Fen; Chou, Wei Yuan; Chou, Tz Chong; Lee, Wei Hwa.

In: Nephron, Vol. 78, No. 4, 1998, p. 440-452.

Research output: Contribution to journalArticle

Chen, A, Sheu, LF, Ho, YS, Lin, YF, Chou, WY, Chou, TC & Lee, WH 1998, 'Experimental focal segmental glomerulosclerosis in mice', Nephron, vol. 78, no. 4, pp. 440-452. https://doi.org/10.1159/000044974
Chen A, Sheu LF, Ho YS, Lin YF, Chou WY, Chou TC et al. Experimental focal segmental glomerulosclerosis in mice. Nephron. 1998;78(4):440-452. https://doi.org/10.1159/000044974
Chen, Ann ; Sheu, Lai Fa ; Ho, Yat Sen ; Lin, Yu Fen ; Chou, Wei Yuan ; Chou, Tz Chong ; Lee, Wei Hwa. / Experimental focal segmental glomerulosclerosis in mice. In: Nephron. 1998 ; Vol. 78, No. 4. pp. 440-452.
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