Exogenous bone marrow cells do not rescue non-irradiated mice from acute renal tubular damage caused by HgCl2, despite establishment of chimaerism and cell proliferation in bone marrow and spleen

T. C. Fang, W. R. Otto, R. Jeffery, T. Hunt, M. R. Alison, H. T. Cook, N. A. Wright, R. Poulsom

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Abstract

Objective: Various studies have shown that bone marrow stem cells can rescue mice from acute renal tubular damage under a conditioning advantage (irradiation or cisplatin treatment) favouring donor cell engraftment and regeneration; however, it is not known whether bone marrow cells (BMCs) can contribute to repair of acute tubular damage in the absence of a selection pressure for the donor cells. The aim of this study was to examine this possibility. Materials and methods: Ten-week-old female mice were assigned into control non-irradiated animals having only vehicle treatment, HgCl 2-treated non-irradiated mice, HgCl2-treated non-irradiated mice infused with male BMCs 1 day after HgCl2, and vehicle-treated mice with male BMCs. Tritiated thymidine was given 1 h before animal killing. Results: Donor BMCs could not alleviate non-irradiated mice from acute tubular damage caused by HgCl2, deduced by no reduction in serum urea nitrogen combined with negligible cell engraftment. However, donor BMCs could home to the bone marrow and spleen and display proliferative activity. This is the first report to show that despite no preparative myeloablation of recipients, engrafted donor BMCs can synthesize DNA in the bone marrow and spleen. Conclusions: Exogenous BMCs do not rescue non-irradiated mice from acute renal tubular damage caused by HgCl2, despite establishment of chimerism and cell proliferation in bone marrow and spleen.

Original languageEnglish
Pages (from-to)592-606
Number of pages15
JournalCell Proliferation
Volume41
Issue number4
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology

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