Exerting the appropriate application of methylprednisolone in acute spinal cord injury based on time course transcriptomics analysis

Liang Yo Yang, Meng Yu Tsai, Shu Hui Juan, Shwu Fen Chang, Chang Tze Ricky Yu, Jung Chun Lin, Kory R. Johnson, Hendrick Gao Min Lim, Yang C. Fann, Yuan Chii Gladys Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Methylprednisolone (MP) is an anti-inflammatory drug approved for the treatment of acute spinal cord injuries (SCIs). However, MP administration for SCIs has become a controversial issue while the molecular effects of MP remain unexplored to date. Therefore, delineating the benefits and side effects of MP and determining what MP cannot cure in SCIs at the molecular level are urgent issues. Here, genomic profiles of the spinal cord in rats with and without injury insults, and those with and without MP treatment, were generated at 0, 2, 4, 6, 8, 12, 24, and 48 h post-injury. A comprehensive analysis was applied to obtain three distinct classes: side effect of MP (SEMP), com-petence of MP (CPMP), and incapability of MP (ICMP). Functional analysis using these genes suggested that MP exerts its greatest effect at 8~12 h, and the CPMP was reflected in the immune re-sponse, while SEMP suggested aspects of metabolism, such as glycolysis, and ICMP was on neurological system processes in acute SCIs. For the first time, we are able to precisely reveal responsive functions of MP in SCIs at the molecular level and provide useful solutions to avoid complications of MP in SCIs before better therapeutic drugs are available.

Original languageEnglish
Article number13024
JournalInternational journal of molecular sciences
Volume22
Issue number23
DOIs
Publication statusPublished - Dec 1 2021

Keywords

  • Gly-colysis
  • Inflammation
  • Methylprednisolone
  • Neurological system
  • Oxidative stress
  • Spinal cord injury
  • Time course transcriptomics

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Exerting the appropriate application of methylprednisolone in acute spinal cord injury based on time course transcriptomics analysis'. Together they form a unique fingerprint.

Cite this