Exercise preconditioning protects against spinal cord injury in rats by upregulating neuronal and astroglial heat shock protein 72

Cheng Kuei Chang, Willy Chou, Hung Jung Lin, Yi Ching Huang, Ling Yu Tang, Mao Tsun Lin, Ching Ping Chang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The heat shock protein 72 (HSP 72) is a universal marker of stress protein whose expression can be induced by physical exercise. Here we report that, in a localized model of spinal cord injury (SCI), exercised rats (given pre-SCI exercise) had significantly higher levels of neuronal and astroglial HSP 72, a lower functional deficit, fewer spinal cord contusions, and fewer apoptotic cells than did non-exercised rats. pSUPER plasmid expressing HSP 72 small interfering RNA (SiRNA-HSP 72) was injected into the injured spinal cords. In addition to reducing neuronal and astroglial HSP 72, the (SiRNA-HSP 72) significantly attenuated the beneficial effects of exercise preconditioning in reducing functional deficits as well as spinal cord contusion and apoptosis. Because exercise preconditioning induces increased neuronal and astroglial levels of HSP 72 in the gray matter of normal spinal cord tissue, exercise preconditioning promoted functional recovery in rats after SCI by upregulating neuronal and astroglial HSP 72 in the gray matter of the injured spinal cord. We reveal an important function of neuronal and astroglial HSP 72 in protecting neuronal and astroglial apoptosis in the injured spinal cord. We conclude that HSP 72-mediated exercise preconditioning is a promising strategy for facilitating functional recovery from SCI.

Original languageEnglish
Pages (from-to)19018-19036
Number of pages19
JournalInternational Journal of Molecular Sciences
Volume15
Issue number10
DOIs
Publication statusPublished - Oct 20 2014

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Keywords

  • Exercise preconditioning
  • Heat shock protein 72
  • Neurological severity score
  • Spinal cord compression

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

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