Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice

You Lan Yang, Gau Jun Tang, Yuh Lin Wu, Huey Wen Yien, Tzong Shyuan Lee, Yu Ru Kou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the effects of mechanical ventilation with a moderately high tidal volume (VT) on acute lung injury (ALI) induced by wood smoke inhalation in anesthetized mice. Animals received challenges of air, 30 breaths of smoke (30SM) or 60 breaths of smoke (60SM) and were then ventilated with a VT of 10 ml/kg (10VT) or 16 ml/kg (16VT). After 4-h mechanical ventilation, the bronchoalveolar-capillary permeability, pulmonary infiltration of inflammatory cells, total lung injury score and pulmonary expressions of interleukin-1β and macrophage inflammatory protein-2 mRNA and proteins in the 30SM + 16VT and 60SM + 16VT groups were greater than those in the 30SM + 10VT and 60SM + 10VT groups, respectively. Additionally, the wet/dry weight ratio of lung tissues and lung epithelial cell apoptosis in the 60SM + 16VT group were greater than those in the 60SM + 10VT group. These differences between the 16VT and 10VT groups were not seen in animals with air challenge. Thus, mechanical ventilation with a moderately high VT in mice exacerbates ALI induced by wood smoke inhalation.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalRespiratory Physiology and Neurobiology
Volume160
Issue number1
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

Fingerprint

Acute Lung Injury
Tidal Volume
Artificial Respiration
Smoke
Lung
Inhalation
Air
Chemokine CXCL2
Capillary Permeability
Lung Injury
Interleukin-1
Epithelial Cells
Apoptosis
Weights and Measures
Messenger RNA

Keywords

  • Acute lung injury
  • Apoptosis
  • Biotrauma
  • Cytokines
  • Mechanical ventilation
  • Smoke inhalation injury

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

Cite this

Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice. / Yang, You Lan; Tang, Gau Jun; Wu, Yuh Lin; Yien, Huey Wen; Lee, Tzong Shyuan; Kou, Yu Ru.

In: Respiratory Physiology and Neurobiology, Vol. 160, No. 1, 01.01.2008, p. 99-108.

Research output: Contribution to journalArticle

Yang, You Lan ; Tang, Gau Jun ; Wu, Yuh Lin ; Yien, Huey Wen ; Lee, Tzong Shyuan ; Kou, Yu Ru. / Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice. In: Respiratory Physiology and Neurobiology. 2008 ; Vol. 160, No. 1. pp. 99-108.
@article{3a82db73b3a84e358062c5b58ccec45b,
title = "Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice",
abstract = "We investigated the effects of mechanical ventilation with a moderately high tidal volume (VT) on acute lung injury (ALI) induced by wood smoke inhalation in anesthetized mice. Animals received challenges of air, 30 breaths of smoke (30SM) or 60 breaths of smoke (60SM) and were then ventilated with a VT of 10 ml/kg (10VT) or 16 ml/kg (16VT). After 4-h mechanical ventilation, the bronchoalveolar-capillary permeability, pulmonary infiltration of inflammatory cells, total lung injury score and pulmonary expressions of interleukin-1β and macrophage inflammatory protein-2 mRNA and proteins in the 30SM + 16VT and 60SM + 16VT groups were greater than those in the 30SM + 10VT and 60SM + 10VT groups, respectively. Additionally, the wet/dry weight ratio of lung tissues and lung epithelial cell apoptosis in the 60SM + 16VT group were greater than those in the 60SM + 10VT group. These differences between the 16VT and 10VT groups were not seen in animals with air challenge. Thus, mechanical ventilation with a moderately high VT in mice exacerbates ALI induced by wood smoke inhalation.",
keywords = "Acute lung injury, Apoptosis, Biotrauma, Cytokines, Mechanical ventilation, Smoke inhalation injury",
author = "Yang, {You Lan} and Tang, {Gau Jun} and Wu, {Yuh Lin} and Yien, {Huey Wen} and Lee, {Tzong Shyuan} and Kou, {Yu Ru}",
year = "2008",
month = "1",
day = "1",
doi = "10.1016/j.resp.2007.09.011",
language = "English",
volume = "160",
pages = "99--108",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier Science Publishers B.V.",
number = "1",

}

TY - JOUR

T1 - Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice

AU - Yang, You Lan

AU - Tang, Gau Jun

AU - Wu, Yuh Lin

AU - Yien, Huey Wen

AU - Lee, Tzong Shyuan

AU - Kou, Yu Ru

PY - 2008/1/1

Y1 - 2008/1/1

N2 - We investigated the effects of mechanical ventilation with a moderately high tidal volume (VT) on acute lung injury (ALI) induced by wood smoke inhalation in anesthetized mice. Animals received challenges of air, 30 breaths of smoke (30SM) or 60 breaths of smoke (60SM) and were then ventilated with a VT of 10 ml/kg (10VT) or 16 ml/kg (16VT). After 4-h mechanical ventilation, the bronchoalveolar-capillary permeability, pulmonary infiltration of inflammatory cells, total lung injury score and pulmonary expressions of interleukin-1β and macrophage inflammatory protein-2 mRNA and proteins in the 30SM + 16VT and 60SM + 16VT groups were greater than those in the 30SM + 10VT and 60SM + 10VT groups, respectively. Additionally, the wet/dry weight ratio of lung tissues and lung epithelial cell apoptosis in the 60SM + 16VT group were greater than those in the 60SM + 10VT group. These differences between the 16VT and 10VT groups were not seen in animals with air challenge. Thus, mechanical ventilation with a moderately high VT in mice exacerbates ALI induced by wood smoke inhalation.

AB - We investigated the effects of mechanical ventilation with a moderately high tidal volume (VT) on acute lung injury (ALI) induced by wood smoke inhalation in anesthetized mice. Animals received challenges of air, 30 breaths of smoke (30SM) or 60 breaths of smoke (60SM) and were then ventilated with a VT of 10 ml/kg (10VT) or 16 ml/kg (16VT). After 4-h mechanical ventilation, the bronchoalveolar-capillary permeability, pulmonary infiltration of inflammatory cells, total lung injury score and pulmonary expressions of interleukin-1β and macrophage inflammatory protein-2 mRNA and proteins in the 30SM + 16VT and 60SM + 16VT groups were greater than those in the 30SM + 10VT and 60SM + 10VT groups, respectively. Additionally, the wet/dry weight ratio of lung tissues and lung epithelial cell apoptosis in the 60SM + 16VT group were greater than those in the 60SM + 10VT group. These differences between the 16VT and 10VT groups were not seen in animals with air challenge. Thus, mechanical ventilation with a moderately high VT in mice exacerbates ALI induced by wood smoke inhalation.

KW - Acute lung injury

KW - Apoptosis

KW - Biotrauma

KW - Cytokines

KW - Mechanical ventilation

KW - Smoke inhalation injury

UR - http://www.scopus.com/inward/record.url?scp=36749034805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36749034805&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2007.09.011

DO - 10.1016/j.resp.2007.09.011

M3 - Article

VL - 160

SP - 99

EP - 108

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 1

ER -