Ex vivo multiphoton analysis of rabbit corneal wound healing following conductive keratoplasty

Tsung Jen Wang, Wen Lo, Chiu Mei Hsueh, Ming-Shium Hsieh, Chen Yuan Dong, Fung Rong Hu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Ex vivo multiphoton imaging is used to characterize rabbit corneal wound healing after conductive keratoplasty (CK) procedures. CK is performed on the right eyes from eight New Zealand albino rabbits while the left eyes are punctured by a keratoplast tip without energy application. Rabbits are humanely sacrificed 1day, 1, 2, and 4weeks after the CK procedure. Eye balls are enucleated and placed on the microscope for multiphoton imaging. Multiphoton imaging reveals damage of corneal epithelium and stroma caused by the CK procedure and the subsequent wound healing process can be followed without histological procedures. Multiphoton excited autofluorescence images demonstrate that re-epithelilialization is accomplished within 1week in both CK and control groups. However, epithelial hyperplasia is observed in CK corneas. In addition, stromal wounds in the control group become inconspicuous within 1week while obvious wounds still exist in CK corneas for at least 4weeks. Postconductive keratoplasty corneal damage and wound healing can be characterized by multiphoton microscopy without histological procedures. Our results suggest that multiphoton microscopy has potential in the clinical evaluation of corneal damage due to refractive surgery, and can be used to study and reduce the unwanted side effects of these procedures.

Original languageEnglish
Article number034019
JournalJournal of Biomedical Optics
Volume13
Issue number3
DOIs
Publication statusPublished - 2008

Keywords

  • collagen
  • conductive keratoplasty
  • corneal wound healing
  • multiphoton microscopy
  • second harmonic generation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

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