Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Tsung-Jen Wang; Wen Lo; Chen Yuan Dong; Fung Rong Hu

doi:10.1117/12.762212

Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Tsung-Jen Wang, Wen Lo, Chen Yuan Dong, Fung Rong Hu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6844
DOIs	https://doi.org/10.1117/12.762212
Publication status	Published - 2008
Event	Ophthalmic Technologies XVIII - San Jose, CA, United States Duration: Jan 19 2008 → Jan 21 2008

Other

Other	Ophthalmic Technologies XVIII
Country/Territory	United States
City	San Jose, CA
Period	1/19/08 → 1/21/08

Keywords

Collagen
Cornea
Multiphoton microscopy
Photorefractive keratectomy (PRK)
Wound healing

ASJC Scopus subject areas

Engineering(all)

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10.1117/12.762212

Cite this

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title = "Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy",

abstract = "The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.",

keywords = "Collagen, Cornea, Multiphoton microscopy, Photorefractive keratectomy (PRK), Wound healing",

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AU - Lo, Wen

AU - Dong, Chen Yuan

AU - Hu, Fung Rong

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Y1 - 2008

N2 - The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

AB - The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

KW - Collagen

KW - Cornea

KW - Multiphoton microscopy

KW - Photorefractive keratectomy (PRK)

KW - Wound healing

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BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

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Y2 - 19 January 2008 through 21 January 2008

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Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Abstract

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Keywords

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