Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Tsung-Jen Wang, Wen Lo, Chen Yuan Dong, Fung Rong Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The aim of this study is to assess the application of multiphoton auto fluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.OD) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6844
DOIs
Publication statusPublished - 2008
EventOphthalmic Technologies XVIII - San Jose, CA, United States
Duration: Jan 19 2008Jan 21 2008

Other

OtherOphthalmic Technologies XVIII
CountryUnited States
CitySan Jose, CA
Period1/19/081/21/08

Fingerprint

Harmonic generation
Microscopic examination
Imaging techniques
Collagen
Surgery
Fluorescence
Fibers
Processing

Keywords

  • Collagen
  • Cornea
  • Multiphoton microscopy
  • Photorefractive keratectomy (PRK)
  • Wound healing

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wang, T-J., Lo, W., Dong, C. Y., & Hu, F. R. (2008). Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6844). [684414] https://doi.org/10.1117/12.762212

Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy. / Wang, Tsung-Jen; Lo, Wen; Dong, Chen Yuan; Hu, Fung Rong.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6844 2008. 684414.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, T-J, Lo, W, Dong, CY & Hu, FR 2008, Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6844, 684414, Ophthalmic Technologies XVIII, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.762212
Wang T-J, Lo W, Dong CY, Hu FR. Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6844. 2008. 684414 https://doi.org/10.1117/12.762212
Wang, Tsung-Jen ; Lo, Wen ; Dong, Chen Yuan ; Hu, Fung Rong. / Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6844 2008.
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