Evolution of pulmonary pathology in severe acute respiratory syndrome

Cheng Hsiang Hsiao, Mu Zong Wu, Chi Long Chen, Po Ren Hsueh, Shun Wen Hsieh, Pan Chyr Yang, Ih Jen Su

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background and Purpose: Severe acute respiratory syndrome (SARS) is characterized by fever with rapid progression to acute respiratory distress and it is associated with substantial morbidity and mortality. Transmission patterns suggest spread by respiratory droplet or close person-to-person contact. To elucidate the correlation of clinical presentation to the pathogenesis and course of the disease, we reviewed the pulmonary pathologic specimens of SARS patients taken at different stages of the disease. Methods: Four "probable" cases of SARS were studied. SARS-associated coronavirus (SARS-CoV) infection was demonstrated using reverse transcriptase-polymerase chain reaction in all 4 patients. The pulmonary specimens were taken on day 7 after symptom onset in patient 1, day 11 in patient 2, day 17 in patient 3, and day 21 in patient 4. Results: The autopsy lung tissue from patient 1, who died 7 days after symptoms onset due to the complication of acute myocardial infarction, revealed mild histologic change in the lung. Only focal pulmonary edema or hemorrhage was seen. In the second patient, who died 11 days after symptom onset, severe acute alveolar damage was characterized by patchy or diffuse lung edema, hyaline membrane formation, and scarce lymphocytic infiltration. The lymphocytes were mostly CD3-positive and CD20-negative. In the third patient, biopsy specimen taken 17 days after symptom onset showed patches of organizing pneumonia with reactive fibroblastic proliferation, more abundant type II pneumocytes and clustering of CD68-positive macrophages within the alveolar spaces. Few CD68-positive syncytial multinucleated giant cells were also seen in the specimens but no viral inclusion body could be identified in these cells. The lung biopsy specimen from patient 4 taken 21 days after symptom onset showed characteristics of the fibrotic stage, with significant myofibroblastic proliferation in the alveolar space and interstitium resulting in loss of pulmonary architecture. The number of CD3-positive lymphocytes and of CD68-positive macrophages in this specimen from a patient in the fibrotic phase of diffuse alveolar damage (DAD) whose condition deteriorated was less than in the specimen from case 3 who was in the early proliferative phase of DAD and eventually recovered. Conclusions: The histologic evolution of SARS coincided with the different stages of DAD: acute, proliferative organizing, and fibrotic stages. SARS cannot be differentiated from the other etiologies of DAD by morphologic examination alone. The absence of DAD does not rule out the possibility of SARS-CoV infection, particularly in the early stage of the disease.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume104
Issue number2
Publication statusPublished - 2005
Externally publishedYes

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Severe Acute Respiratory Syndrome
Pathology
Lung
Viral Inclusion Bodies
Lymphocytes
Alveolar Epithelial Cells
Biopsy
Coronavirus
Hyalin
Alveolar Macrophages
Pulmonary Edema
Giant Cells
Infection
Reverse Transcriptase Polymerase Chain Reaction
Lung Diseases
Cluster Analysis
Autopsy
Edema
Pneumonia
Fever

Keywords

  • Pathology
  • Pulmonary alveoli
  • SARS virus
  • Severe acute respiratory syndrome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hsiao, C. H., Wu, M. Z., Chen, C. L., Hsueh, P. R., Hsieh, S. W., Yang, P. C., & Su, I. J. (2005). Evolution of pulmonary pathology in severe acute respiratory syndrome. Journal of the Formosan Medical Association = Taiwan yi zhi, 104(2), 75-81.

Evolution of pulmonary pathology in severe acute respiratory syndrome. / Hsiao, Cheng Hsiang; Wu, Mu Zong; Chen, Chi Long; Hsueh, Po Ren; Hsieh, Shun Wen; Yang, Pan Chyr; Su, Ih Jen.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 104, No. 2, 2005, p. 75-81.

Research output: Contribution to journalArticle

Hsiao, CH, Wu, MZ, Chen, CL, Hsueh, PR, Hsieh, SW, Yang, PC & Su, IJ 2005, 'Evolution of pulmonary pathology in severe acute respiratory syndrome', Journal of the Formosan Medical Association = Taiwan yi zhi, vol. 104, no. 2, pp. 75-81.
Hsiao, Cheng Hsiang ; Wu, Mu Zong ; Chen, Chi Long ; Hsueh, Po Ren ; Hsieh, Shun Wen ; Yang, Pan Chyr ; Su, Ih Jen. / Evolution of pulmonary pathology in severe acute respiratory syndrome. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 2005 ; Vol. 104, No. 2. pp. 75-81.
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AU - Wu, Mu Zong

AU - Chen, Chi Long

AU - Hsueh, Po Ren

AU - Hsieh, Shun Wen

AU - Yang, Pan Chyr

AU - Su, Ih Jen

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N2 - Background and Purpose: Severe acute respiratory syndrome (SARS) is characterized by fever with rapid progression to acute respiratory distress and it is associated with substantial morbidity and mortality. Transmission patterns suggest spread by respiratory droplet or close person-to-person contact. To elucidate the correlation of clinical presentation to the pathogenesis and course of the disease, we reviewed the pulmonary pathologic specimens of SARS patients taken at different stages of the disease. Methods: Four "probable" cases of SARS were studied. SARS-associated coronavirus (SARS-CoV) infection was demonstrated using reverse transcriptase-polymerase chain reaction in all 4 patients. The pulmonary specimens were taken on day 7 after symptom onset in patient 1, day 11 in patient 2, day 17 in patient 3, and day 21 in patient 4. Results: The autopsy lung tissue from patient 1, who died 7 days after symptoms onset due to the complication of acute myocardial infarction, revealed mild histologic change in the lung. Only focal pulmonary edema or hemorrhage was seen. In the second patient, who died 11 days after symptom onset, severe acute alveolar damage was characterized by patchy or diffuse lung edema, hyaline membrane formation, and scarce lymphocytic infiltration. The lymphocytes were mostly CD3-positive and CD20-negative. In the third patient, biopsy specimen taken 17 days after symptom onset showed patches of organizing pneumonia with reactive fibroblastic proliferation, more abundant type II pneumocytes and clustering of CD68-positive macrophages within the alveolar spaces. Few CD68-positive syncytial multinucleated giant cells were also seen in the specimens but no viral inclusion body could be identified in these cells. The lung biopsy specimen from patient 4 taken 21 days after symptom onset showed characteristics of the fibrotic stage, with significant myofibroblastic proliferation in the alveolar space and interstitium resulting in loss of pulmonary architecture. The number of CD3-positive lymphocytes and of CD68-positive macrophages in this specimen from a patient in the fibrotic phase of diffuse alveolar damage (DAD) whose condition deteriorated was less than in the specimen from case 3 who was in the early proliferative phase of DAD and eventually recovered. Conclusions: The histologic evolution of SARS coincided with the different stages of DAD: acute, proliferative organizing, and fibrotic stages. SARS cannot be differentiated from the other etiologies of DAD by morphologic examination alone. The absence of DAD does not rule out the possibility of SARS-CoV infection, particularly in the early stage of the disease.

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KW - Pathology

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