Evodiamine inhibits in vitro angiogenesis: Implication for antitumorgenicity

Kou Gi Shyu, Shankung Lin, Chun Chung Lee, Eden Chen, Lie Chwen Lin, Bao Wei Wang, Shiow Chwen Tsai

Research output: Contribution to journalArticle

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Abstract

Evodiamine, the major bioactive compound isolated from Chinese herbal drug named Wu-Chu-Yu, has been reported to exhibit anti-tumor growth and metastasis. However, the effect of evodiamine on angiogenesis remains to be investigated. We used the fresh medium containing evodiamine or human lung adenocarcinoma cell (CL1 cells) derived conditioned media free of evodiamine to test their capability to induce in vitro angiogenesis, i.e., human umbilical vein endothelial cells (HUVECs) tube formation and invasion. We demonstrated that evodiamine could directly inhibit in vitro HUVECs tube formation and invasion. Locally administered evodiamine also inhibited the in vivo angiogenesis in the chick embryo chorioallantoic membrane (CAM) assay. The gene expression of vascular endothelial growth factor (VEGF) and the p44/p42 mitogen-activated protein kinase (MAPK, ERK) that correlated with endothelial cells angiogenesis were inhibited by evodiamine. We found that the evodiamine-treated CL1 cells derived conditioned medium showed decreased VEGF release and reduced ability of inducing in vitro tube formation. After the collection of conditioned media, the VEGF expression of remaining CL1 cells were determined by Western analyses and revealed that evodiamine decreased VEGF expression. Moreover, administration of recombinant human VEGF165 (rhVEGF165) induced tube formation and ERK phosphorylation by HUVECs, and partially attenuated inhibitory effect of evodiamine. From these results, we suggested that evodiamine is a potent inhibitor of angiogenesis. The mechanism might involve at least the inhibition of VEGF expression, probably through repression of ERK phosphorylation.

Original languageEnglish
Pages (from-to)2234-2243
Number of pages10
JournalLife Sciences
Volume78
Issue number19
DOIs
Publication statusPublished - Apr 4 2006

Fingerprint

Vascular Endothelial Growth Factor A
Endothelial cells
Human Umbilical Vein Endothelial Cells
Conditioned Culture Medium
Phosphorylation
Chinese Herbal Drugs
In Vitro Techniques
evodiamine
Chorioallantoic Membrane
Angiogenesis Inhibitors
Mitogen-Activated Protein Kinase 1
Chick Embryo
Gene expression
Tumors
Assays
Endothelial Cells
Neoplasm Metastasis
Membranes
Gene Expression
Growth

Keywords

  • Angiogenesis
  • ERK
  • Evodiamine
  • HUVECs
  • VEGF

ASJC Scopus subject areas

  • Pharmacology

Cite this

Shyu, K. G., Lin, S., Lee, C. C., Chen, E., Lin, L. C., Wang, B. W., & Tsai, S. C. (2006). Evodiamine inhibits in vitro angiogenesis: Implication for antitumorgenicity. Life Sciences, 78(19), 2234-2243. https://doi.org/10.1016/j.lfs.2005.09.027

Evodiamine inhibits in vitro angiogenesis : Implication for antitumorgenicity. / Shyu, Kou Gi; Lin, Shankung; Lee, Chun Chung; Chen, Eden; Lin, Lie Chwen; Wang, Bao Wei; Tsai, Shiow Chwen.

In: Life Sciences, Vol. 78, No. 19, 04.04.2006, p. 2234-2243.

Research output: Contribution to journalArticle

Shyu, KG, Lin, S, Lee, CC, Chen, E, Lin, LC, Wang, BW & Tsai, SC 2006, 'Evodiamine inhibits in vitro angiogenesis: Implication for antitumorgenicity', Life Sciences, vol. 78, no. 19, pp. 2234-2243. https://doi.org/10.1016/j.lfs.2005.09.027
Shyu KG, Lin S, Lee CC, Chen E, Lin LC, Wang BW et al. Evodiamine inhibits in vitro angiogenesis: Implication for antitumorgenicity. Life Sciences. 2006 Apr 4;78(19):2234-2243. https://doi.org/10.1016/j.lfs.2005.09.027
Shyu, Kou Gi ; Lin, Shankung ; Lee, Chun Chung ; Chen, Eden ; Lin, Lie Chwen ; Wang, Bao Wei ; Tsai, Shiow Chwen. / Evodiamine inhibits in vitro angiogenesis : Implication for antitumorgenicity. In: Life Sciences. 2006 ; Vol. 78, No. 19. pp. 2234-2243.
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