Evodiamine induces transient receptor potential vanilloid-1-mediated protective autophagy in U87-MG astrocytes

Ann Jeng Liu, Sheng Hao Wang, Sz Ying Hou, Chien Ju Lin, Wen Ta Chiu, Sheng Huang Hsiao, Thay Hsiung Chen, Chwen Ming Shih

Research output: Contribution to journalArticle

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Abstract

Cerebral ischemia is a leading cause of mortality and morbidity worldwide, which results in cognitive and motor dysfunction, neurodegenerative diseases, and death. Evodiamine (Evo) is extracted from Evodia rutaecarpa Bentham, a plant widely used in Chinese herbal medicine, which possesses variable biological abilities, such as anticancer, anti-inflammation, antiobesity, anti-Alzheimer's disease, antimetastatic, antianoxic, and antinociceptive functions. But the effect of Evo on ischemic stroke is unclear. Increasing data suggest that activation of autophagy, an adaptive response to environmental stresses, could protect neurons from ischemia-induced cell death. In this study, we found that Evo induced autophagy in U87-MG astrocytes. A scavenger of extracellular calcium and an antagonist of transient receptor potential vanilloid-1 (TRPV-1) decreased the percentage of autophagy accompanied by an increase in apoptosis, suggesting that Evo may induce calcium-mediated protective autophagy resulting from an influx of extracellular calcium. The same phenomena were also confirmed by a small interfering RNA technique to knock down the expression of TRPV1. Finally, Evo-induced c-Jun N-terminal kinases (JNK) activation was reduced by a TRPV1 antagonist, indicating that Evo-induced autophagy may occur through a calcium/c-Jun N-terminal kinase (JNK) pathway. Collectively, Evo induced an influx of extracellular calcium, which led to JNK-mediated protective autophagy, and this provides a new option for ischemic stroke treatment.

Original languageEnglish
Article number354840
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
Publication statusPublished - 2013

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Autophagy
Astrocytes
Calcium
JNK Mitogen-Activated Protein Kinases
Evodia
Stroke
Aptitude
Herbal Medicine
evodiamine
vanilloid receptor subtype 1
Brain Ischemia
Neurodegenerative Diseases
Small Interfering RNA
Alzheimer Disease
Cell Death
Phosphotransferases
Ischemia
Apoptosis
Inflammation
Morbidity

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Evodiamine induces transient receptor potential vanilloid-1-mediated protective autophagy in U87-MG astrocytes. / Liu, Ann Jeng; Wang, Sheng Hao; Hou, Sz Ying; Lin, Chien Ju; Chiu, Wen Ta; Hsiao, Sheng Huang; Chen, Thay Hsiung; Shih, Chwen Ming.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 354840, 2013.

Research output: Contribution to journalArticle

Liu, Ann Jeng ; Wang, Sheng Hao ; Hou, Sz Ying ; Lin, Chien Ju ; Chiu, Wen Ta ; Hsiao, Sheng Huang ; Chen, Thay Hsiung ; Shih, Chwen Ming. / Evodiamine induces transient receptor potential vanilloid-1-mediated protective autophagy in U87-MG astrocytes. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
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