Evidence of independent origin of multiple tumors from patients with prostate cancer

Liang Cheng, Sang Yong Song, Thomas G. Pretlow, Fadi W. Abdul-Karim, Hsing Jien Kung, Deborah V. Dawson, Won Sang Park, Young Wan Moon, Min Lung Tsai, W. Marston Linehan, Michael R. Emmert-Buck, Lance A. Liotta, Zhengping Zhuang

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalJournal of the National Cancer Institute
Volume90
Issue number3
DOIs
Publication statusPublished - Feb 4 1998
Externally publishedYes

Fingerprint

Prostatic Neoplasms
Neoplasms
Loss of Heterozygosity
Prostate
Microsatellite Repeats
Chromosomes
BRCA1 Gene
DNA
Tumor Suppressor Genes
Paraffin
Formaldehyde
Carcinogenesis
Alleles

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cheng, L., Song, S. Y., Pretlow, T. G., Abdul-Karim, F. W., Kung, H. J., Dawson, D. V., ... Zhuang, Z. (1998). Evidence of independent origin of multiple tumors from patients with prostate cancer. Journal of the National Cancer Institute, 90(3), 233-237. https://doi.org/10.1093/jnci/90.3.233

Evidence of independent origin of multiple tumors from patients with prostate cancer. / Cheng, Liang; Song, Sang Yong; Pretlow, Thomas G.; Abdul-Karim, Fadi W.; Kung, Hsing Jien; Dawson, Deborah V.; Park, Won Sang; Moon, Young Wan; Tsai, Min Lung; Linehan, W. Marston; Emmert-Buck, Michael R.; Liotta, Lance A.; Zhuang, Zhengping.

In: Journal of the National Cancer Institute, Vol. 90, No. 3, 04.02.1998, p. 233-237.

Research output: Contribution to journalArticle

Cheng, L, Song, SY, Pretlow, TG, Abdul-Karim, FW, Kung, HJ, Dawson, DV, Park, WS, Moon, YW, Tsai, ML, Linehan, WM, Emmert-Buck, MR, Liotta, LA & Zhuang, Z 1998, 'Evidence of independent origin of multiple tumors from patients with prostate cancer', Journal of the National Cancer Institute, vol. 90, no. 3, pp. 233-237. https://doi.org/10.1093/jnci/90.3.233
Cheng, Liang ; Song, Sang Yong ; Pretlow, Thomas G. ; Abdul-Karim, Fadi W. ; Kung, Hsing Jien ; Dawson, Deborah V. ; Park, Won Sang ; Moon, Young Wan ; Tsai, Min Lung ; Linehan, W. Marston ; Emmert-Buck, Michael R. ; Liotta, Lance A. ; Zhuang, Zhengping. / Evidence of independent origin of multiple tumors from patients with prostate cancer. In: Journal of the National Cancer Institute. 1998 ; Vol. 90, No. 3. pp. 233-237.
@article{e8c6ea296c0b48b9b48a4aaeabb05ad2,
title = "Evidence of independent origin of multiple tumors from patients with prostate cancer",
abstract = "Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.",
author = "Liang Cheng and Song, {Sang Yong} and Pretlow, {Thomas G.} and Abdul-Karim, {Fadi W.} and Kung, {Hsing Jien} and Dawson, {Deborah V.} and Park, {Won Sang} and Moon, {Young Wan} and Tsai, {Min Lung} and Linehan, {W. Marston} and Emmert-Buck, {Michael R.} and Liotta, {Lance A.} and Zhengping Zhuang",
year = "1998",
month = "2",
day = "4",
doi = "10.1093/jnci/90.3.233",
language = "English",
volume = "90",
pages = "233--237",
journal = "Journal of the National Cancer Institute",
issn = "0027-8874",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Evidence of independent origin of multiple tumors from patients with prostate cancer

AU - Cheng, Liang

AU - Song, Sang Yong

AU - Pretlow, Thomas G.

AU - Abdul-Karim, Fadi W.

AU - Kung, Hsing Jien

AU - Dawson, Deborah V.

AU - Park, Won Sang

AU - Moon, Young Wan

AU - Tsai, Min Lung

AU - Linehan, W. Marston

AU - Emmert-Buck, Michael R.

AU - Liotta, Lance A.

AU - Zhuang, Zhengping

PY - 1998/2/4

Y1 - 1998/2/4

N2 - Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.

AB - Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.

UR - http://www.scopus.com/inward/record.url?scp=0032481322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032481322&partnerID=8YFLogxK

U2 - 10.1093/jnci/90.3.233

DO - 10.1093/jnci/90.3.233

M3 - Article

VL - 90

SP - 233

EP - 237

JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

SN - 0027-8874

IS - 3

ER -