Evidence for the reversibility of cellular DNA lesion induced by mammalian topoisomerase II poisons

Y. H. Hsiang, Leroy-Fong Liu

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Like many intercalative antitumor drugs, the nonintercalative antitumor drug epipodophyllotoxin VM-26 (teniposide) induces topoisomerase II-linked DNA breaks as revealed by cell lysis with a strong protein denaturant such as sodium dodecyl sulfate or alkali. We show that the majority of topoisomerase II-linked DNA breaks reflect the formation of reversible topoisomerase II-DNA cleavable complexes in drug-treated cells by demonstrating the reversibility of this unusual type of DNA damage at elevated temperatures (e.g. 65°C).

Original languageEnglish
Pages (from-to)9713-9715
Number of pages3
JournalJournal of Biological Chemistry
Volume264
Issue number17
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Type II DNA Topoisomerase
Poisons
Teniposide
DNA Breaks
Antineoplastic Agents
DNA
Podophyllotoxin
Alkalies
Sodium Dodecyl Sulfate
DNA Damage
Cells
Temperature
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Evidence for the reversibility of cellular DNA lesion induced by mammalian topoisomerase II poisons. / Hsiang, Y. H.; Liu, Leroy-Fong.

In: Journal of Biological Chemistry, Vol. 264, No. 17, 1989, p. 9713-9715.

Research output: Contribution to journalArticle

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