Evidence for the reversibility of cellular DNA lesion induced by mammalian topoisomerase II poisons

Y. H. Hsiang, Leroy-Fong Liu

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Like many intercalative antitumor drugs, the nonintercalative antitumor drug epipodophyllotoxin VM-26 (teniposide) induces topoisomerase II-linked DNA breaks as revealed by cell lysis with a strong protein denaturant such as sodium dodecyl sulfate or alkali. We show that the majority of topoisomerase II-linked DNA breaks reflect the formation of reversible topoisomerase II-DNA cleavable complexes in drug-treated cells by demonstrating the reversibility of this unusual type of DNA damage at elevated temperatures (e.g. 65°C).

Original languageEnglish
Pages (from-to)9713-9715
Number of pages3
JournalJournal of Biological Chemistry
Volume264
Issue number17
Publication statusPublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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